检验医学与临床Issue(7)：937-939,3.DOI:10.3969/j.issn.1672-9455.2015.07.025
新型心肌梗死大鼠模型制备方法的建立和模型鉴定
Establishment and assessment of rat model of myocardial infarction
摘要
Abstract
Objective To establish a new ,fast and efficient preparation method of myocardial infarction model . Methods Sprague‐Dawley rats were anesthetized by using isoflurane gas ,then myocardial infarction models were prepared by ligation of ramus descendens anterior arteriae coronaria sinistrae ,after bluntly separation of intercostal muscle .Sham operation rat models were prepared by punctuation ,not ligation ,of ramus descendens anterior arteriae coronaria sinistrae .Small animal electrocardiograph ,hemodynamic analyzer ,2 ,3 ,5‐triphenyltetrazolium chloride (TTC) staining and hematoxylin‐eosin (HE) staining were used to identify myocardial infarction models .Results Survival rates of myocardial infarction models and sham operation models were both 80 .0% .Electrocardiograph anal‐ysis indicated that ST segment was raised apparently in myocardial infarction models on 2 and 7 days after operation . Hemodynamic detection indicated that diastolic blood pressure ,systolic blood pressure ,left ventricular systolic pres‐sure and max ascending and descending rate of left ventricular pressure in myocardial infarction models were lower than sham operation models ,but heart rate and heart ventricle end diastolic pressure were higher (P < 0 .05) .TTC staining showed that the color of infracted myocardium was white ,which was obviously different with normal myo‐cardium ,HE staining showed that infracted cardiac muscle cells were swelling ,disorderly arranged ,with cavity or e‐ven break ,and inflammatory cell infiltration could be found within intercellular spaces of fractured cardiac muscle cells .Conclusion Preparation method ,constructed in this research ,could be efficient and easily performed to estab‐lish myocardial infarction models .关键词
心肌梗死/动物模型/大鼠/冠状动脉Key words
myocardial infarction/experimental model/rat/coronary artery引用本文复制引用
裴之俊,李伏燕,陈义加,王俊杰,李薇,吴瑞敏,杨怡..新型心肌梗死大鼠模型制备方法的建立和模型鉴定[J].检验医学与临床,2015,(7):937-939,3.基金项目
国家自然科学青年基金资助项目（81401447）。 （）
