检验医学与临床Issue(8)：1034-1036,3.DOI:10.3969/j.issn.1672-9455.2015.08.004
云南汉族人群 Tim-3启动子区-1516G＞ T 位点多态性与系统性红斑狼疮关联性研究
Association analysis of Tim-3-1516G>T polymorphism with systemic lupus erythematosus in a han population of Yun-nan Province
摘要
Abstract
Objective The aim of the present study was to analyze the association between the Tim‐3 polymor‐phism‐1516G> T and susceptibility to systemic lupus erythematosus(SLE) in a group of Han population from Yun‐nan province .Methods Polymerase chain reaction‐restriction fragment length polymorphism analysis (PCR‐RFLP) was used to detect the polymorphism of‐1516G> T site in 132 SLE patients and 120 controls .and we used indirect immunofluorescence assay and liner immunoblotting to detect three related aotuantibodies of SLE ,which include the anti‐double stranded DNA(dsDNA)antibody ,the anti‐Sm antibody and the anti‐RNP antibody .We used direct count‐ing method to calculate the genotype and allele frequencies to conduct the Hardy‐Weinberg equilibrium test ,and we usedχ2 test to compare the difference of the genotypic and allelic frequencies between SLE and control groups and the correlation of autoantibodies and genotypes .Results We found there are statistically significant differences in geno‐type and allele frequency of‐1516G> T between SLE and control group(P<0 .05);The genotype(GG ,GT ,TT)fre‐quency in the SLE group is 0 .818 2 ,0 .181 8 and 0 .000 0 ,which in the control group is 0 .916 7 ,0 .075 0 and 0 .008 3 respectively .Conclusion The‐1516 site of Tim‐3 gene has SNP variations ,the‐1516G> T genotype maybe related with the susceptibility to SLE in a Han population of Yunnan province ,but different genotypes may not affect the ex‐pression of related autoantibodies of SLE .关键词
系统性红斑狼疮/T细胞免疫球蛋白域黏蛋白域蛋白-3/基因多态性/遗传易感性Key words
SLE/Tim-3/gene polymorphism/susceptibility引用本文复制引用
高辉,彭传梅,付晓野,曹向红,董玉琳,王杨..云南汉族人群 Tim-3启动子区-1516G＞ T 位点多态性与系统性红斑狼疮关联性研究[J].检验医学与临床,2015,(8):1034-1036,3.基金项目
云南省科技厅-昆明医科大学联合专项项目（2011FB232）。 （）
