检验医学与临床Issue(8)：1045-1047,3.DOI:10.3969/j.issn.1672-9455.2015.08.008
HCV 早期感染者病毒载量与 CD4＋CD25＋Foxp3＋调节性 T 淋巴细胞相关性研究
The correlation study between viral load in the early HCV-infected patients and CD4+CD25+Foxp3+ regulatory T cells
摘要
Abstract
Objective To study the correlation between HCV viral load in the early HCV‐infected patients(an‐tigen‐positive antibody‐negative) and CD4+ CD25+ Foxp3+ regulatory T cells ,and to know the function of CD4+CD25+ Foxp3+ regulatory T lymphocytes in the HCV infections .Methods We will use the real‐time quantitative polymerase chain reaction(FQ RT‐PCR) technique to detect HCV RNA viral load in 22 cases of early infected pa‐tients with HCV core positive antigen negative antibody(positive HCV Ag ,negative HCV Ab) ,26 cases of chronic HCV‐infected patients with core positive antigen and positive antibody (positive HCV Ag ,positive HCV Ab) ,24 ca‐ses of following HCV‐infected patients with core negative antigen positive antibody (negative HCV Ag ,positive HCV Ab) and 22 cases healthy controls .We also will use flow cytometry technique to detect the level of CD4+ CD25+Foxp3+ regulatory T lymphocytes of nucleated cells in peripheral blood in each group .Results The CD4+ CD25+Foxp3+ regulatory T lymphocytes in the group of positive HCV Ag negative HCV Ab ,the group of positive HCV Ag positive HCV Ab ,the group of negative HCV Ag positive HCV Ab and healthy control group is step‐down ,of which the group of original positive antibody negative antigen (4 .86 ± 2 .14)% is higher than other groups(3 .74 ± 1 .67)% ,(2 .74 ± 1 .27)% ,(2 .56 ± 1 .18)% .The difference is statistically significant(t values were 2 .023 ,4 .125 , 4 .402 ,P<0 .05);CD4+CD25+ Foxp3+ regulatory T lymphocytes and HCV RNA viral load logarithmic (copy/mL , Ig) were positively correlated(r=0 .535 ,P<0 .05) in core positive antigen negative antibody group .Conclusion The level of CD4+CD25+ Foxp3+ regulatory T lymphocytes in HCV early infections is associated with the set‐point of high virus .CD4+CD25+ Foxp3+ regulatory T lymphocytes level may be one of the factors to speed up the early HCV infection .关键词
HCV/早期感染/调节性T淋巴细胞/病毒载量/相关性Key words
HCV/early infection/regulatory T lymphocytes/viral load/relevance引用本文复制引用
黄启强,黄丽庆,周青,肖寒,黄伙荣,胡淑燕..HCV 早期感染者病毒载量与 CD4＋CD25＋Foxp3＋调节性 T 淋巴细胞相关性研究[J].检验医学与临床,2015,(8):1045-1047,3.基金项目
广东省医学科研基金课题（A2013647）。 （）
