检验医学与临床Issue(8)：1048-1050,3.DOI:10.3969/j.issn.1672-9455.2015.08.009
外源性一氧化碳释放分子2对大鼠脊髓缺血再灌注损伤的保护作用研究
Protective effects of exogenous carbon monoxide releasing molecules 2 in rat with spinal cord ischemia and reperfusion in-jury
摘要
Abstract
Objective To investigate the protective effects of exogenous carbon monoxide releasing molecules 2 (CROM2) in rat with spinal cord ischemia and reperfusion injury .Methods A total of 30 healthy male Wistar rats were divided into experiment group ,control group and blank group ,with 10 rats in each group .Rats of experiment group were given exogenous CORM2 by intravenous injection 1 h before modeling .Same operation was performed in experiment group and control group to construct rat models .Rats in control group were injected the same dose of sa‐line 1 h after the construction of models .Rat in blank group were given only abdominal operation without any other treatment ,and were injected the same dose of saline 1 h after operation .At different time point of operation ,evalua‐tion of Tarlov scale ,histopathology examination of myeloid tissue ,and detection of interleukin‐6(IL‐6) and tumor necrosis factor‐α(TNF‐α) were performed .Results At different time point of reperfusion ,Tarlov scores of experi‐ment group were higher than control group ,but those of the two groups were lower than blank group (P<0 .05) .At 48 h after ischemia‐reperfusion ,damage of neuron in control group was obvious ,which was not found in blank group , and the characteristics of neuron damage in experiment group were between control group and blank group .At 48 h after ischemia‐reperfusion ,levels of IL‐6 and TNF‐αin experiment group and control group were significantly higher than blank group ,while those in experiment group were significantly lower than control group (P<0 .05) .Conclusion Exogenous CORM2 could be with protective effects of spinal cord ischemia‐reperfusion injury ,which might be de‐pendent on the inhibition of the releasing of inflammatory cytokines .关键词
外源性一氧化碳释放分子2/脊髓缺血再灌注损伤/白细胞介素-6/肿瘤坏死因子-αKey words
exogenous carbon monoxide releasing molecules 2/spinal cord ischemia-reperfusion injury/interleukin-6/tumor necrosis factor-α引用本文复制引用
刘志刚,熊敏,余化龙,曾云..外源性一氧化碳释放分子2对大鼠脊髓缺血再灌注损伤的保护作用研究[J].检验医学与临床,2015,(8):1048-1050,3.基金项目
湖北省十堰市科技局基金支持项目［十科发（2013）20号］。 （）
