检验医学与临床Issue(9)：1196-1197,2.DOI:10.3969/j.issn.1672-9455.2015.09.007
阿托伐他汀对高糖诱导的系膜细胞凋亡的影响
Effect of atorvastatin on apoptosis of hyperglycemia induced mesangial cells in rat
摘要
Abstract
Objective To investigate the influence of different concentrations of atorvastatin on apoptosis of rat mesangial cells .Methods Rat immortal mesangial cells were cultured in vitro and divided into 5 groups:control (C) group ,hyperglycemia (30 mmol/L ,H)group ,hyperglycemia (30 mmol/L)+ atorvastatin(10-7 mmol/L)(H+A1) group ,hyperglycemia (30 mmol/L)+ atorvastatin(10-6 mmol/L)(H + A2)group and hyperglycemia (30 mmol/L)+atorvastatin(10-5 mmol/L)(H+ A3)group .Apoptosis was detected by Annexin V‐FITC and PI double staining ,and the caspase‐3 activity was assayed by the caspase‐3 activity kit .Results Compared with the group C , the mesangial cells apoptosis rate and caspase‐3 activity in the group H were significantly increased ,the differences were statistically significant(P<0 .05) .Compared with the group H ,the mesangial cells apoptosis rate and caspase‐3 activity in the H+ A1 ,2 ,3 groups were significantly decreased ,the differences were statistically significant ( P<0 .05) ,moreover which showing the concentration dependence .Conclusion Atorvastatin may inhibit aldosterone in‐duced mesangial cells apoptosis via caspase pathway .关键词
阿托伐他汀/高糖/系膜细胞/凋亡/caspase-3Key words
atorvastatin/high glucose/mesangial cells/apoptosis/caspase-3引用本文复制引用
朱丹,胡莹,赵欣,马吉芳,郝建兵,任连生,崔蕾..阿托伐他汀对高糖诱导的系膜细胞凋亡的影响[J].检验医学与临床,2015,(9):1196-1197,2.基金项目
黑龙江省教育厅科学技术研究项目（12521293）。 （）
