检验医学与临床Issue(9):1215-1217,3.DOI:10.3969/j.issn.1672-9455.2015.09.014
3株导管相关血流感染鲍曼不动杆菌同源性分析
Analysis on homology of 3 strains of Acinetobacter bnaumannii in catheter-related bloodstream infection
摘要
Abstract
Objective To analyze the homology of 3 strains of Acinetobacter baumannii isolated from different bloodstream positions in the same patient for definiting the laboratory diagnosis of central venous catheter‐related bloodstream infection at the gene level to provide the basis for clinical prevention and treatment .Methods 3 strains of Acinetobacter baumannii were identified and the drug sensitivity was performed by using Microscan Walkaway 40Plus microbiological idetification and drug susceptibility system(Sienems ,German) .The homogeneous genotyping was performed by using enterobacteria repetitive intergenic consensus‐polymerase chain reaction (ERIC‐PCR) and Agarose gel electrophoresis .Results The positive products were amplified from 3 strains of Acinetobacter bauman‐nii .13 bands with the same location appeared by electrophoresis .Conclusion 3 strains of Acinetobacter baumannii belong to the same genotype .ERIC‐PCR can be used in the homology analysis of Acinetobacter baumannii .关键词
中心静脉导管/导管相关血流感染/鲍曼不动杆菌/同源性Key words
central venous catheter/catheter-related bloodstream infection/acinetobacter baumanni/ho-mology引用本文复制引用
孙恒彪,陈佑明,张婧,潘祖汉,谢俊杰..3株导管相关血流感染鲍曼不动杆菌同源性分析[J].检验医学与临床,2015,(9):1215-1217,3.基金项目
广东省广州市科技计划项目(2012Y5-00004)。 ()