检验医学与临床Issue(10)：1369-1371,1373,4.DOI:10.3969/j.issn.1672-9455.2015.10.013
耐碳青霉烯类肠杆菌科细菌表型检测及其耐药性研究
Research on the phenotype and drug resistance of carbapenem-resistant Enterobacteriaceae
摘要
Abstract
Objective To explore the phenotype and drug resistance of carbapenem‐resistant Enterobacteriace‐ae(CRE) .Methods Minimal inhibitory concentration(MIC) method was used to analyze the drug resistance of En‐terobacteriaceae .Kirby‐Bauer (Ertapenem) was used to screen the carbapenemases strains .Improving Hodge experi‐ment ,the one with adding boric acid and Kirby‐Bauer compounding oxacillin and EDTA‐Imipenem were used to con‐firmed the phenotype .Results The drug resistance rate of Enterobacteriacea to imipenem (IPM ) and meropenem (MEM) were 2 .7% and 2 .2% ,in 11 pcs screening positive by Kirby‐Bauer (ETP) ,3 pcs improved Hodge tested positive ,1 pic carbapenemases tested by phenotype ,2 pcs ESBLs or AmpC enzyme .2 pcs weekly positive tested AmpC ,TEM ,and CTX‐M gene ,not carbapenemases .The blaIMP gene were tested .improved Hodge positive plants . Conclusion The corporation of improved Hodge experiment ,the one with adding boric acid and Kirby‐Bauer com‐pounding oxacillin and EDTA‐Imipenem has good performance on CRE testing .关键词
肠杆菌科细菌/碳青霉烯酶表型/耐药性分析Key words
Enterobacteriaceae/carbapenemases/analysis of drug resistance引用本文复制引用
万芳,陈恒,柯茂彬,崔敏涛,鲁艳,李永联,吴学诗..耐碳青霉烯类肠杆菌科细菌表型检测及其耐药性研究[J].检验医学与临床,2015,(10):1369-1371,1373,4.基金项目
广东省佛山市顺德区医学科研项目（2013091）。 （）
