检验医学与临床Issue(12)：1681-1683,3.DOI:10.3969/j.issn.1672-9455.2015.12.009
实时荧光定量PCR检测宫腔脱落细胞中P27kip1及cylinE基因的表达及意义
Real-time quantitative PCR in determination of P27kip1 and cyclin E expression in ntrauterine exfoliated cells and its clin- ical relevance
摘要
Abstract
Objective To dectect the expression of P27kip1 and cyclin E genes in intrauterine exfoliated cells of endometrial cancer and normal people and explore its clinical relevance in diagnosing and prognostic monitoring . Methods 80 cases about intrauterine exfoliated cells of the endometrial cancer patients and normal people were col‐lected and extracted the total RNA .And the target genes were detected by the real‐time quantitative PCR and calcu‐late the data through the method of ΔΔCt .Then compare the expression of P27kip1 and cyclin E genes in endometrial cancer patient and normal people .All the experimental data were statistically analyzed by SPSS17 .0 software .Results The real‐time quantitative PCR dedicated that the expression of P27kip1 gene in normal people was higher than en‐dometrial cancer patients(P<0 .05) .With the increasing degree of malignancy ,the expression of P27kip1 was signifi‐cantly reduced(P<0 .05) .The expression of cyclin E gene was presented a negative correlation with P27kip1 .Conclu‐sion Real‐time fluorescent quantitative PCR can sensitively and specifically detected the expression of P27kip1 and cy‐clin E in the intrauterine exfoliate cells .There has a close relationship between the expression of P27kip1 and cyclin E and the development of endometrial cancer .The research has provide us some theory and data support of the diagno‐sing and monitoring of endometrial cancer .关键词
实时荧光定量PCR/子宫内膜癌/P27kip1/cyclin E/早期诊断Key words
real-time quantitative PCR/endometrial cancer/P27kip1 gene/cyclin E gene/early diagnosis引用本文复制引用
李菊香,胡巍,车团结,潘云燕,曹玲,惠方,葛万文,杨佩琦,沈明辉,张尚弟,常运朝..实时荧光定量PCR检测宫腔脱落细胞中P27kip1及cylinE基因的表达及意义[J].检验医学与临床,2015,(12):1681-1683,3.基金项目
甘肃省科学计划项目（113JHTA003）；兰州市科学计划项目（2010-1-143）。 （）
