摘要
Abstract
Objective To investigate the effects of vitamin D3 on liver pathology and fibrotic profiles ,acute liver injury was produced in BALB/C mice by CCl4 .Methods Twenty five BALB/C mice were randomly and equally divided into five groups:control group ,vehicle group ,and three vitamin D3 groups (n=5 for each group) .Doses of vitamin D3 were 15 μg ,7 .5 μg ,1 μg in the vitamin D3 groups (daily ,intraperitoneal) ,which were indicated as high , medium and low does .Mice in control group and vehicle group were daily intraperitoneal injected with 0 .9% Nacl .Af‐ter the two weeks treatment ,vehicle group and treatment groups were intraperitoneal injected with 0 .1% CCl4 in olive (0 .2 mL/2 d ,ip) 7 days later .Meanwhile ,the control group was equivalently intraperitoneal injected with 0 .9% Nacl daily .At the end of administration of CCl4 solution ,blood and liver tissue were harvested from all the mice .Pathologi‐cal changes in liver tissues were analyzed by HE staining ,reticular fiber staining and Masson staining .Alanine amin‐otransferase (ALT) and Aspartate transaminase (AST ) were detected with routine biochemical method .Hyauline (HA) ,collagen typeⅣ (IVC) ,laminin(LN) ,and collagen typeⅢ N‐terminal peptide (PⅢ NP) were detected with RIA .Results The levels of liver index ,ALT ,AST ,HA ,LN ,PⅢ NP ,CⅣ were significantly higher in the vehicle group than that in the control group (all P<0 .01) .Compared with vehicle group ,the levels of serum liver index , ALT ,AST ,HA ,LN ,PⅢNP ,CⅣ were significantly debased after the treatment with vitamin D3 (all P<0 .01) .Con‐clusion Vitamin D3 has anti‐inflammation and anti‐fibrosis effects in the mice experimental liver injuries induced by CCl4 ,by reducing level of serum fibrosis in early liver fibrosis .关键词
维生素D3/肝损伤/肝纤维化Key words
vitamin D3/experimental hepatic injury/liver fibrosis
