检验医学与临床Issue(12)：1720-1722,3.DOI:10.3969/j.issn.1672-9455.2015.12.024
内质网应激在大鼠重症急性胰腺炎肝损伤的临床意义
The funcation of endoplasmic reticulum stress in rat severe acute pancreatitis-associated liver injury
李杨 1赵鹏 1苏春 1陈建立 1刘洋 1张国志1
作者信息
- 1. 河北联合大学附属医院，河北唐山 063000
- 折叠
摘要
Abstract
Objective To observe the changes of endoplasmic reticulum stress‐related molecules glucose‐regu‐lated protein 78 (GRP78) and CCAAT/enhancerbinding protein homologous protein (CHOP) in the severe acute pancreatitis‐associated liver injury and to discuss whether endoplasmic reticulum stress (ERS) participate in the pro‐gress of severe acute pancreatitis‐associated liver injury .Methods The subject established the model of severe acute pancreatitis‐associated liver injury by 5% sodium taurocholate .80 male SD rats were randomly divided into sham groups(n=40)and model groups(n=40) .Then each groups were randomly divided into 3h ,6h ,12h ,24h and 36h ac‐cording time points .The changes of serum amylase (AMY) ,alanine aminotransferase (ALT) and aspartate amin‐otransferase (AST) were detected by automatic biochemical analyzer .Pathological changes of the liver were observed with HE staining .The expression of GRP78 and CHOP were detected by Western blot .Results (1)The level of ser‐um enzyme were significantly increased and reached the peak value at 12h in model groups(P<0 .05) .The level of serum enzyme in the model groups were significantly increased compared with corresponding time points of sham groups (P<0 .05) .(2)The HE staining showed that the injury of the liver was the most serious at 24 h .(3)Western blot test showed that the expression of GRP78 and CHOP in the model groups were significantly increased and reached the peak value at 24 h(P<0 .05) .The expression of GRP78 and CHOP in the model groups were significant‐ly increased compared with corresponding time points of sham groups(P<0 .05) .Conclusion The injury of the liver was the most serious at 24h and ERS may be involved in the progress of severe acute pancreatitis‐associated liver in‐jury .关键词
内质网应激/葡萄糖调节蛋白78/CCAAT/增强子结合蛋白同源蛋白/重症急性胰腺炎/肝损伤Key words
endoplasmic reticulum stress/glucose-regulated protein 78/CCAAT/enhancerbinding protein homologous protein/severe acute pancreatitis/liver injury引用本文复制引用
李杨,赵鹏,苏春,陈建立,刘洋,张国志..内质网应激在大鼠重症急性胰腺炎肝损伤的临床意义[J].检验医学与临床,2015,(12):1720-1722,3.
