检验医学与临床Issue(14)：2034-2036,3.DOI:10.3969/j.issn.1672-9455.2015.14.024
慢性阻塞性肺疾病急性加重期 HBP 与 CD64的表达及意义
Expression and significance of HBP and CD64 in acute exacerbation of chronic obstructive pulmonary disease
江雁琼 1贺端明 1伍慧妍1
作者信息
- 1. 广州医科大学附属第五医院，广州 510700
- 折叠
摘要
Abstract
Objective To investigate the expression and significance of CD64 and neutrophils heparin binding protein(HBP)in acute exacerbation of chronic obstructive pulmonary disease(COPD).Methods 200 inpatients and outpatients with COPD in the respiration department of our hospital from July 2013 to November 2014 were collected as the research subjects,including 100 cases of stable COPD and 100 cases of acute exacerbation COPD (AECOPD). CD64 expression was measured by using flow cytometry and immunofluorescence,the expression of HBP was detec-ted by enzyme-linked immunosorbent assay(ELISA),meanwhile serum procalcitonin (PCT),high-sensitivity C-reac-tive protein (hs-CRP)and white blood cell(WBC)count were detected for conducting the comparative analysis.The diagnostic value of HBP and CD64 in AECOPD was evaluated.In AECOPD patients,the sputum bacterial culture was performed and the nasal swab was conducted the PCR respiratory virus detection for determining pathogens.The HBP and CD64 expressions in AECOPD caused by different etiologies were compared.Results The expressions of HBP and CD64 in AECOPD were (86.84 ±38.91 )ng/mL and (6.85 ±2.82)MFI,which were significantly higher than(56.24 ± 24.58)ng/mL and (3.32 ± 1.09 )MFI in stable COPD,the differences were statistically significant (P <0.01).In different etiologies of AECOPD,the HBP and CD64 expressions in bacterial infection and mixed infec-tion were significantly higher than those in viral infection and non-infection,the differences were statistically signifi-cant(P <0.01).The susceptibility and specificity of HBP and CD64 to bacterial infection were more than 85% and more than 90%,which were significantly higher than those to mixed infection and viral infection.Conclusion HBP and CD64 can be used as the assisted diagnostic markers of AECOPD.关键词
慢性阻塞性肺疾病/急性加重期/肝素结合蛋白/CD64Key words
COPD/acute exacerbation/HBP/CD64引用本文复制引用
江雁琼,贺端明,伍慧妍..慢性阻塞性肺疾病急性加重期 HBP 与 CD64的表达及意义[J].检验医学与临床,2015,(14):2034-2036,3.
