检验医学与临床Issue(14)：2088-2089,2092,3.DOI:10.3969/j.issn.1672-9455.2015.14.048
Grubbs 法在酶联免疫吸附试验乙型肝炎两对半室内质控的应用探讨
Study on application of Grubbs method in internal quality control for hepatitis B determination by ELISA
伍启康 1陈斌鸿 1陈展泽 1郑雪莲 1戴伟良1
作者信息
- 1. 广东省佛山市第一人民医院检验科 528000
- 折叠
摘要
Abstract
Objective To investigate the feasibility of the Grubbs method combined with the ±3 s,±2 s and 7 T rule applied to the temporary internal quality control method for hepatitis B determination by ELISA.Methods The continuous 20 batches of internal quality control data in the hepatitis B quantitative determination by ELISA at the same quality control batch number were consulted and the application of Grubbs method combined with the ±3 s,±2 s and 7 T rule was statistically analyzed.Results The 20 times detection values in the HBsAg,HbsAb and HbeAg quantitative determination by ELISA were in the controlled state;the 20 times detection values displayed by the Grubbs method in the HbeAb quantitative determination were in the controlled state,but the thirteenth detection results violated the ±2 s rule,which was judged as a warning condition,its data were retained and paid attention,the reasons were actively analyzed to avoid the subsequent determination being warning or out of control again;the 20 times detection values in the HbcAb quantitative determination by ELISA were in the controlled state.Conclusion The Grubbs method combined with the ±3 s,±2 s and 7 T rule can be used as the temporary internal quality control method for hepatitis B determination by ELISA.关键词
Grubbs 法/酶联免疫吸附试验/室内质控Key words
Grubbs method/ELISA/internal quality control引用本文复制引用
伍启康,陈斌鸿,陈展泽,郑雪莲,戴伟良..Grubbs 法在酶联免疫吸附试验乙型肝炎两对半室内质控的应用探讨[J].检验医学与临床,2015,(14):2088-2089,2092,3.
