口腔颌面外科杂志Issue(5)：341-344,4.DOI:10.3969/j.issn.1005-4979.2014.05.004
Erk1/2信号通路在上颌突间充质细胞成骨分化中的调控作用
Erk1/2 Signaling Pathway Regulates Osteogenesis of Maxillary Primordium Mesenchymal Cells
丁月峰 1王学娟1
作者信息
- 1. 江苏省苏州市吴江区口腔诊所，江苏 苏州 215200
- 折叠
摘要
Abstract
Objective:To investigate the effect of Erk1/2 signal pathway on the osteogenic differentiation of maxillary pri-mordium mesenchymal cells in vitro. Methods: Maxillary primordium mesenchymal cells (MPMCs) were obtained from E13.5 embryos and cultured in vitro. Cells of the second passage were cultured in the osteogenic medium. 10 nmol/L PD98059 (an inhibitor of the ERK1/2 signaling pathway) was added in the medium in experimental group for 7 days. The immunofluorescence technique, Alizarin red S staining, and qPCR were applied to evaluate the osteogenic capability of MPMCs. Results: The E13.5 MPMCs was successfully cultured and passaged in vitro. Osteogenic induction enhanced the phosphorylation of Erk1/2. Inhibition of the phosphorylation of Erk1/2 resulted in down regulation of the expression of ALP, Runx2,OCN, and decreasing the formation of calcium nodules in MPMCs. Conclusion: The Erk1/2 signal pathway plays crucial roles on the osteogenic differentiation of MPMCs in vitro.关键词
Erk1/2/上颌突/间充质细胞/成骨分化/小鼠Key words
Erk1/2/maxillary primordium/mesenchymal cells/osteogenic differentiation/rats分类
医药卫生引用本文复制引用
丁月峰,王学娟..Erk1/2信号通路在上颌突间充质细胞成骨分化中的调控作用[J].口腔颌面外科杂志,2014,(5):341-344,4.
