口腔颌面外科杂志Issue(3)：167-171,5.DOI:10.3969/j.issn.1005-4979.2015.03.002
条件培养液诱导牙龈间充质干细胞分化的体外实验研究
Investigation of Multipotent of Gingival Mesenchymal Stem Cells Induced by Apical Tooth Germ Cell-conditioned Medium
摘要
Abstract
Objective:To establish an indirect co-culture system of rat apical tooth germ-conditioned medium(APTG-CM) and gingival mesenchymal stem cells (GMSCs) for investigating differentiation potential in vitro, in order to find a new seed cell for periodontal tissue engineering. Methods: Using the limiting dilution technique, single-colony derived human GMSCs were isolated and expanded to obtain homogeneous populations. Alkaline phosphatase (ALP) activity, mineral-ization behavior, gene expression of cementoblast phenotype (OCN, BSP, ALP, COL-1, CP23) were evaluated in vitro. Results: GMSCs were capable of forming colonies and osteogenic and adipogenic differentiations under induction. The induced GMSCs exhibited several characteristics of cementoblast lineages, as indicated by the morphological changes, in-creased proliferation, high ALP activity and the expression of cementum-related genes in vitro. Conclusion: GMSCs could obtain the ability of mineralization, and apical tooth germ cell-conditioned medium may promote gingival mesenchymal stem cells differentiation.关键词
人类/牙龈间充质干细胞/增殖/细胞分化Key words
human/gingival mesenchymal stem cells(GMSCs)/proliferation/differentiation分类
医药卫生引用本文复制引用
陈岩,冯妍慧芝,刘宏伟..条件培养液诱导牙龈间充质干细胞分化的体外实验研究[J].口腔颌面外科杂志,2015,(3):167-171,5.基金项目
国家自然科学基金(30772418、81271152)；上海市科学技术委员会科研计划(074119514) （30772418、81271152）
