兰州大学学报（医学版）Issue(2)：46-49,54,5.DOI:10.13885/j.issn.1000-2812.2015.02.009
超快速液相色谱-质谱/质谱联用法测定氯丙嗪制剂中氯丙嗪含量
Ultra faster method for the quantification of chlorpromazine in chlorpromazine preparation using UFLC-MS/MS
摘要
Abstract
Objective To establish a simple and ultra-fast method for the quantification of chlorpromazine in chlorpromazine preparation by liquid chromatography/tandem mass spectrometric (UFLC-MS/MS) method. Methods Sample preparation consisted of extraction by adding ethanol, filtration with microporous membrane and centrifugation of the sample subsequently. LC-MS/MS detection was performed by using a triple-stage quadrupole mass spectrometer working in multiple reaction monitoring mode with positive electrospray ion-ization, using the transitions of m/z 319.0→85.8 of chlorpromazine. Chromatographic separation was achieved using a 3.0 mm × 75 mm, 2.0μm Shim-pack XR-ODS column with isocratic flow and elution with acetonitrile-water-formic acid ( 90∶10∶0.02, V/V/V ) and a flow-rate of 0.4 mL/min. The analyte was quanti-fied in a single run within 3 mins. Results Linearity was demonstrated over the expected concentration range 1~256 ng/mL. The lower limit of quantification was 1 ng/mL, respectively. Intra-day precision expressed as relative standar deviation was 1.34% or less, while inter-day result was 1.89% or less for chlorpromazine. Conclusion The method has good repeatability, high sensitivity and more convenient, and can be successfully applied for the determination of chlorpromazine in chlorpromazine preparation.关键词
氯丙嗪/制剂/超快速液相色谱-质谱/质谱联用法Key words
chlorpromazine/preparation/ultra-fast liquid chromatography/tandem mass spectrometric分类
医药卫生引用本文复制引用
徐丽婷,张娟红,孙爱军,王荣,谢华,张汝学,张强,黄小玉,贾正平..超快速液相色谱-质谱/质谱联用法测定氯丙嗪制剂中氯丙嗪含量[J].兰州大学学报（医学版）,2015,(2):46-49,54,5.基金项目
科技部重大专项（2008ZXJ09004-52）；甘肃省科技重大专项 （）
