兰州大学学报（医学版）Issue(2)：50-54,5.DOI:10.13885/j.issn.1000-2812.2015.02.010
大黄素诱导对内质网应激相关蛋白表达及肝癌HepG2细胞凋亡的影响
Emodin induces endoplasmic reticulum stress-associated apoptosis in hepatocellular carcinoma HepG2 cell line
摘要
Abstract
Objective To investigate the effect of emodin on the expression of endoplasmic reticulum stress (ERS)-associated proteins in hepatocellular carcinoma HepG2 cells. Methods After treatment with various concentrations of emodin(0, 10, 50, 100μmol/L), tumor cell viability and apoptosis were measured by MTT (methyl thiazolyl tetrazolium )and flow cytometry assays, respectively. The GRP78 (glucose-regulated pro-tein 78), CHOP(C/EBP homologous protein), caspase-12 were analysed by Western Blotting. Results Emodin inhibited the proliferation of HepG2 cells, promoted their apoptosis in the concentration-depandent manner and enhanced the expression of ERS chaperone GRP78 in HepG2 cells. Emodin treament also increased the expression of the two known essential ERS-associated apoptotic proteins, CHOP and caspase-12 and elevated caspase-12 activation in HepG2 cells. Conclusion Emodin induces apoptosis in hepatocellular carcinoma HepG2 cells probably involving the induction of ERS associated proteins.关键词
大黄素/增殖/内质网应激/HepG2细胞Key words
Emodin/proliferation/endoplasmic reticulum stress/HepG2 cells分类
医药卫生引用本文复制引用
党中峰,党雅梅,陈城,蔡伟,郭应芳,杨华,那光伟,何科基..大黄素诱导对内质网应激相关蛋白表达及肝癌HepG2细胞凋亡的影响[J].兰州大学学报（医学版）,2015,(2):50-54,5.基金项目
甘肃省中医药管理局科研项目 （）
