兰州大学学报（医学版）Issue(3)：8-13,6.DOI:10.13885/j.issn.1000-2812.2015.03.002
微小RNA-320a通过靶向FoxM1调控肝癌细胞迁移
MicroRNA-320a regulate migration of liver cancer cells by targeting FoxM1
摘要
Abstract
Objective To investigate whether MicroRNA-320a (miR-320a) play a role in regulating the migra-tion of hepatocellular carcinoma cell by targeting FoxM1and provide a new target for liver cancer treatment. Methods Bioinformatics tool, microRNA.org was used to find the microRNA which can targeted FoxM1. Western blot and dual luciferase reporter experiments was employed to test the regulation of miR-320a on FoxM1. Transwell chamber was utilized to analyze the effects of miR-320a on the migration of hepatocellular carcinoma cells and analyze the functional relevance between miR-320a and FoxM1 in this process. Results Bioinformatics analysis showed that miR-320a has two potential binding sites in the 3'untranslated region of FoxM1, and the Western blot results showed miR-320a reduced FoxM1 protein levels in liver cancer cells, and double-luciferase reporter experiments further demonstrated that miR-320a could directly regulate FoxM1 expression in post-transcriptional manner. miR-320a mimics can downregulate the expression of FoxM1 (P<0.01), and miR-320a inhibitor can upregulate the expression of FoxM1 (P<0.05). Transwell experiments indicated that miR-320a mimic significantly inhibited the migration of hepatocellular carcinoma cell line-Huh7 and HCC-LM3, on the contrary, miR-320a inhibitor can significantly promoted the migration of hepato-cellular carcinoma cell line. In addition, the functions of miR-320a mimic and inhibitor on liver cancer cells were significantly weakened, when FoxM1 was downregulated by small interfering RNA in these cell lines. Conclusion miR-320a suppresses the migration of liver cancer cells by directly targeting oncogene FoxM1.关键词
肝癌/微小RNA-320a/FoxM1/细胞迁移Key words
hepatocellular carcinoma/microRNA-320a/FoxM1/cell migration分类
医药卫生引用本文复制引用
叶小娟,蔡慧,何斌,高勇..微小RNA-320a通过靶向FoxM1调控肝癌细胞迁移[J].兰州大学学报（医学版）,2015,(3):8-13,6.基金项目
上海市医学重点专科建设资助项目（ZK2012A26）；上海市浦东新区卫生系统重点学科群建设项目（PWZxq2014-04）；上海市浦东新区卫生系统领先人才培养计划项目 （）
