兰州大学学报（医学版）Issue(3)：29-32,4.DOI:10.13885/j.issn.1000-2812.2015.03.006
两种硫辛酸对映体对胚胎体外发育的影响
Effects of two kinds of lipoic acid enantiomers on early embryo development in vitro
摘要
Abstract
Objective To investigate the effects of (R)-and (S)-lipoic acid on early embryonic development. Methods The thawed and recoveried embryos of cleavage stage were cultured into GⅡmedium droplets, con-taining different density of (R)-and (S)-lipoic acid, then compared the morulaes rate, densification rate and blastocysts rate between two group in vitro. Results Lipoic acid could increase developmental rate of embryo in vitro. Morulaes, densification, blastocysts and high quality blastocysts rate of 10 × 10-9 mol/m3 (R)-lipoic acid treated group were 59.2%, 55.1%, 49.0%and 54.2%, respectively, which were all higher than those in the other tested groups (P<0.01). The effect of (R)-and (S)-lipoic acid would weakened following the increased concentration. Conclusion Lipoic acid has a promoting effect on the development of embryos in vitro, espe-cially the effects on embryo morulaes and blastocysts rate, among them the (R)-lipoic acid 10 × 10-9 mol/m3 group is the most efficient one.关键词
硫辛酸/胚胎发育/胚胎培养液/氧化应激Key words
lipoic acid/early embryonic development/embryo culture media/oxidative stress分类
医药卫生引用本文复制引用
景原雪,薛石龙,王乃辉,李红星,孙亮,宋德潇..两种硫辛酸对映体对胚胎体外发育的影响[J].兰州大学学报（医学版）,2015,(3):29-32,4.基金项目
国家自然科学基金青年基金项目（81200469）；甘肃省科技厅研究技术与开发专项 （）
