临床肝胆病杂志Issue(9)：906-909,4.DOI:10.3969/j.issn.1001-5256.2014.09.017
非酒精性脂肪性肝病生物样本库的建立与管理
Establishment and management of nonalcoholic fatty liver disease biobank
摘要
Abstract
Objective To investigate the collection and preservation of blood specimens from patients with nonalcoholic fatty liver disease (NAFLD)and the establishment and information management of biobank.Methods Whole blood samples were collected from 1226 pa-tients who were diagnosed with NAFLD based on B-mode ultrasound and blood tests from October 2009 to October 2013.Biochemical pa-rameters were measured.Plasma and whole-blood genomic DNA was extracted from the samples,and the purity and concentration of DNA were determined.Specimens were preserved in a refrigerator (-80℃).An information management system for NAFLD biobank was estab-lished.Results Specimens of 1226 NAFLD patients,including those of 83 twins and 100 families,were collected.The success rate was 100% for extraction of plasma and whole-blood genomic DNA.One hundred DNA samples were randomly selected for testing,and the re-sults showed that the collected specimens met the requirements of following experiments.Conclusion The NAFLD Biobank has been suc-cessfully established in this study.It has the standard information management system and enables the quality control and information man-agement of specimens,laying a solid foundation for further research on NAFLD.关键词
脂肪肝/生物标本库/信息存储和检索Key words
fatty liver/biological specimen banks/information storage and retrieval分类
医药卫生引用本文复制引用
陈立震,张海英,姜曼,牛同红,王健,耿宁,刘昊刚,辛永宁,宣世英..非酒精性脂肪性肝病生物样本库的建立与管理[J].临床肝胆病杂志,2014,(9):906-909,4.基金项目
国家自然科学基金资助项目 （）
