陈启明 1袁宏 1戴丽蓉 2蒋妮 1任文婷 2陈青锋1
作者信息
- 1. 兰州大学第一医院，兰州 730000
- 2. 兰州大学基础医学院，兰州 730000
- 折叠
摘要
Abstract
Objective To evaluate the mental disorders in mice with thioacetamide (TAA)-induced acute hepatic encephalopathy (AHE). Methods Thirty male Kunming mice were equally and randomly divided into two groups (treatment group and control group).Mice in the treatment group received an intraperitoneal injection of TAA (200 mg·kg -1 ·d -1 for 3 days,up to 600 mg·kg -1 ),as compared with the same volume of physiological saline in the control group.Neurological function score,light/dark box,open field,and elevated plus -maze test were used to determine behavioral parameters at 24 h after the model was established.Serum ammonia,aspartate amino transferase (AST),al-anine aminotransferase (ALT),and total bilirubin (TBil)were measured at 24 h post determination of behavioral parameters.Comparison be-tween two groups was made by independent -samples t test.Results In the light/dark box test,the treatment group had significantly reduced residence time in the light box and number of shuttles between two boxes (t =-4.006,P <0.01;t =-2.656,P <0.05);in the open field test,the treatment group had significantly reduced central distance,central time,and number of vertical movements (t =-3.639,P <0.05;t =-2.294,P <0.05;t =-2.282,P <0.05),as well as increased grooming time (t =5.992,P <0.01);meanwhile,the number of open-arm entries and residence time in the open arm for the treatment group were also reduced in the elevated plus -maze test,as compared with the control group (t =-3.584,P <0.05;t =-3.992,P <0.05).However,the total distance in the open field test showed no significant difference between the two groups (t =-0.96,P >0.05).Moreover,compared with the control group,the treatment group had significant-ly higher levels of serum ammonia (t =-3.168,P <0.05),ALT (t =4.316,P <0.05),AST (t =-2.581,P <0.05),and TBil (t =-9.127,P <0.01).Conclusion Mice with AHE induced by an intraperitoneal injection of low -dose TAA have anxiety -related behav-iors;the anxiety -related behaviors are not associated with reduced locomotor activities.关键词
肝性脑病/焦虑/硫代乙酰胺Key words
hepatic encephalopathy/anxiety/thioacetamide分类
医药卫生
