临床肝胆病杂志Issue(4)：541-545,5.DOI:10.3969/j.issn.1001-5256.2015.04.016
慢性乙型肝炎患者外周血中T淋巴细胞亚群和CD4+CD25+调节性T淋巴细胞的表达水平与HBV DNA定量的相关分析
Correlation analysis of percentages of T lymphocyte subsets and CD4 +CD25 +regulatory T cells and HBV DNA level in peripheral blood of chronic hepatitis B patients
摘要
Abstract
Objective To investigate the correlation between the percentages of T lymphocyte subsets (CD3 +T,CD4 +T,CD8 +T,and CD4 +/CD8 +T)and CD4 +CD25 +regulatory T cells (Treg cells)and hepatitis B virus (HBV)DNA level in the peripheral blood of chro-nic hepatitis B (CHB)patients.Methods We chose 175 patients with HBV infection,who visited The Second People′s Hospital of Lanzhou from July to December,2012.The patients were divided into CHB group (n=63)group and asymptomatic carrier (ASC)group (n=1 12).In the same period,50 healthy examinees served as control group.The percentages of CD3 +,CD4 +,CD8 +,and CD4 +/CD8 +T lymphocytes and CD4 +CD25 +Treg cells in the serum of three groups were measured by flow cytometry.HBV DNA level was ana-lyzed by quantitative real-time PCR.The comparison of means between multiple groups was performed by analysis of variance.Further comparison between any two means was performed by LSD-t test.Correlation analysis was performed by Pearson′s correlation test.Results The percentage of CD4 +CD25 +Treg (30.97%±18.78%)in the peripheral blood of CHB patients was significantly higher than that of the control group (27.30%±17.59%)(P<0.05).Compared with the control group,the CHB and ASC groups had significantly reduced percentages of CD3 +and CD4 +T lymphocytes and CD4 +/CD8 +T lymphocyte ratio (P<0.05)and a significantly increased percentage of CD8 +T lymphocytes (P<0.05).There was a positive correlation between the percentage of CD4 +CD25 +Treg cells and HBV DNA level in the ASC and CHG groups (r=0.501 and 0.682,P<0.05 for both).Conclusion There are normal percentages of T lymphocyte sub-sets in HBV infection patients.HBV DNA can lead to an increased percentage of CD4 +CD25 +Treg cells,indicating that the imbalance of CD4 +CD25 +Treg and CD4 +/CD8 +T cells plays an important role in the chronic progression of hepatitis B.关键词
肝炎,乙型,慢性/T淋巴细胞,调节/T淋巴细胞亚群/病毒载量Key words
hepatitis B,chronic/T-lymphocytes,regulatory/T-lymphocyte subsets/viral buralen分类
医药卫生引用本文复制引用
李彩东,陈锡莲,田鹏飞,李惠军,吴斌..慢性乙型肝炎患者外周血中T淋巴细胞亚群和CD4+CD25+调节性T淋巴细胞的表达水平与HBV DNA定量的相关分析[J].临床肝胆病杂志,2015,(4):541-545,5.基金项目
兰州市科技发展指导性计划项目 （）
