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多元复合酶降解黄姜薯蓣皂苷元的工艺研究

李文君 王成章 张水晶 鲁黎

生物质化学工程Issue(4):23-27,5.
生物质化学工程Issue(4):23-27,5.DOI:10.3969/j.issn.1673-5854.2014.04.005

多元复合酶降解黄姜薯蓣皂苷元的工艺研究

Application of Multivariate Composite Enzymatic Hydrolysis in Extraction of the Diosgenin from Yellow Ginger

李文君 1王成章 1张水晶 1鲁黎2

作者信息

  • 1. 中国林业科学研究院林产化学工业研究所,江苏 南京 210042
  • 2. 南京林业大学南方学院,江苏 南京 210037
  • 折叠

摘要

Abstract

This paper focused on the impact factors of enzymatic hydrolysis by applying multivariate composite enzyme in the process.The results indicated that the quaternary multivariate composite enzyme, i.e.alkaline pectinase +tannase +hemicellulase+β-glycosidase,had the best degradation effect.The highest yield of diosgenin was 4.34 %,when the condition was 1.5 % compound enzyme of turmeric under 50 ℃,pH value 6.5,solid-liquid ratio of 1∶4 and ethanol as extraction solvent for 10 h.Compared with the traditional acid hydrolysis which yield was only 1.61 %,application of multivariate composite enzyme hydrolysis can improve the highest yield of diosgenin to 1 .69 times.

关键词

黄姜/薯蓣皂苷元/多元复合酶/酶解

Key words

yellow ginger/diosgenin/multivariate composite enzyme/enzymatic hydrolysis

分类

化学化工

引用本文复制引用

李文君,王成章,张水晶,鲁黎..多元复合酶降解黄姜薯蓣皂苷元的工艺研究[J].生物质化学工程,2014,(4):23-27,5.

基金项目

国家高科技研究发展计划(863项目SS2014AA021802);国际合作项目 ()

生物质化学工程

OA北大核心

1673-5854

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