生物质化学工程Issue(2)：12-16,5.DOI:10.3969/j.issn.1673-5854.2015.02.003
高效液相色谱-内标法测定焦性没食子酸含量
Determination of Pyrogallol Using Reversed-phase HPLC and Internal Standard Method
摘要
Abstract
A rapid, specific, and accurate HPLC method was developed and validated for the assay of pyrogallol. The assay involved a gradient elution of pyrogallol with a Thermo Hypersil C18 column (250 mm × 4. 6 mm,5 μm) using mobile phase composition of methanol and water (0. 1% TFA) at a flow rate of 1. 0 mL/min. UV detection was performed at 266 nm. Ellagic acid was used as an internal standard. The retention time of pyrogallol and ellagic acid was 4. 33 and 19. 25 min,respectively. The linearity range of pyrogallol concentration was from 62. 5 to 2 000 mg/L. The fitting equation of calibration curve was y =0. 000 138 66x+0. 001 92 (R=0. 999 7) and a mean recovery of 103. 6% was found. The proposed method was applied for the quantitative determination of pyrogallol in pyrogallol products.关键词
焦性没食子酸/没食子酸/反相高效液相色谱法/内标法Key words
pyrogallol/ellagic acid/RP-HPLC/internal standard method分类
化学化工引用本文复制引用
张亮亮,汪咏梅,徐曼,陈笳鸿..高效液相色谱-内标法测定焦性没食子酸含量[J].生物质化学工程,2015,(2):12-16,5.基金项目
国家863计划资助(2014AA021802) （2014AA021802）
