林产化学与工业Issue(3)：130-134,5.DOI:10.3969/j.issn.0253-2417.2014.03.022
高速及高分辨率原子力显微镜用于研究Trichoderma reesei纤维素酶在结晶纤维素上的行为
The Action of Trichoderma reesei Cellulases on Crystalline Cellulose with High Speed and High Resolution Atomic Force Microscopy
王芳芳 1黄峰1
作者信息
- 1. 山东大学微生物技术国家重点实验室，山东 济南 250100
- 折叠
摘要
Abstract
The advantages of the atomic force microscopy and high speed atomic force microscopy on studying the dynamics of bio-macromolecule and the cellulytic enzyme system of Trichoderma reesei were presented. The results of high speed atomic force microscopy and high resolution atomic force microscopy in investigating the actions of Cel7A(TrCel7A), Cel6A(TrCel6A) and Cel7B(TrCel7B) of Trichoderma reesei on crystalline cellulose were reviewed in detail. The conclusion reveals that the loading of crystalline cellulose into catalytic domain and the hydrolysis of the glucosidic bond are prerequisite for the movement of Cel7A;increasing the hydrophobic area of crystalline cellulose and the combination of Cel6A and Cel7A enhance the hydrolytic rate of crystalline cellulose;Cel7B makes crystalline cellulose swelling. And it is prospective that HP-AFM will play an important role in elucidating the acting mechanism of cellulase on crystalline cellulose.关键词
高速原子力显微镜/纤维素酶/结晶纤维素Key words
high speed atomic force microscopy/cellullase/crystalline cellulose分类
化学化工引用本文复制引用
王芳芳,黄峰..高速及高分辨率原子力显微镜用于研究Trichoderma reesei纤维素酶在结晶纤维素上的行为[J].林产化学与工业,2014,(3):130-134,5.
