林产化学与工业Issue(5)：127-132,6.DOI:10.3969/j.issn.0253-2417.2014.05.021
米根霉无载体固定化发酵产L-乳酸
Production of L-Lactic Acid by Self-immobilized Rhizopus oryzae
摘要
Abstract
The main factors affecting self-immobilization of Rhizopus oryzae NLX-M-1 for L-lactic acid production were studied in present study. The optimum fermentation conditions utilizing wood fiber-based glucose for R. oryzae self-immobilization were obtained. The optimal results were achieved as 100 g/L initial glucose, 2 g/L ( NH4 ) 2 SO4 , 2%inoculum size ( value fraction) , 30 g/L CaCO3 , 0. 1 g/L KH2 PO4 , 0. 25 g/L MgSO4·7H2 O and 0. 1 g/L ZnSO4·7H2 O. Under this optimal condition, R. oryzae formed pellets with an average diameter of about 1 mm during the fermentation, the production of L-lactic acid was 76. 6 g/L and the conversion rate was 81. 6 %. R. oryzae in acid explosive residue hydrolyzate containing 60 g/L glucose became 1. 2 mm pellets, and produced 36. 4 g/L L-lactic acid with a conversion rate of 63. 5 %.关键词
米根霉/无载体固定化/L-乳酸/玉米秸秆/酶解葡萄糖Key words
Rhizopus oryzae/self-immobilize/L-lactic acid/corn stover/acid explosive residue分类
化学化工引用本文复制引用
陈晓佩,张丽,顾夕梅,李鑫,勇强,余世袁..米根霉无载体固定化发酵产L-乳酸[J].林产化学与工业,2014,(5):127-132,6.基金项目
江苏省自然科学基金项目(BK20131426) （BK20131426）
国家自然科学基金资助项目(31000278) （31000278）
教育部高等学校博士学科点专项科研基金项目(20103204120009) （20103204120009）
