临床口腔医学杂志Issue(12)：716-717,718,3.DOI:10.3969/j.issn.1003-1634.2014.12.006
人白介素－1β水平与人牙周膜细胞OPG表达关系
Relationship between the level of rhIL-1βand osteoprotegerin expression in human periodontal ligament cells
张侬 1谢妮 2付云 3舒睿 1杨雅1
作者信息
- 1. 深圳市龙岗区妇幼保健院口腔科 广东 深圳 518172
- 2. 深圳大学第一附属医院中心实验室 广东 深圳 518035
- 3. 中山大学光华口腔医学院牙周科 广东 广州 510055
- 折叠
摘要
Abstract
Objective:To determine the effects of rhIL-1βon osteoprotegerin expression of cultured human periodon-tal ligament cells (HPDLCs) in vitro,and reach the role of rhIL-1βon the HPDLCs during their osteogenic differentiation. Method:HPDLCs were derived from periodontal membrane of healthy premolars that were extracted for orthodontics and cultured in vitro and secretion levels of the OPG mRNA expression were determined by ELISA and RT-PCR methods under the effect of rhIL-1βat different concentrations (0、5、10、15μg/L). Result:ELISA and RT-PCR assay showed that rhIL-1βact on HPDLCs reduced the OPG protein secretion and mRNA expression in the 5、10、15 μg/L groups,with negative positively correlated with concentrations. As compared with 0 μg/L control group at the same time,the difference was sig-nificant(P<0.05) in the 5、10、15μg/L groups. Conclusion:rhIL-1βcan inhibition the OPG expression in HPDLCs at ex-perimental concentration and play an adverse role in the alveolar bone reconstruction.关键词
牙周膜成纤维细胞/重组人白介素-1β/细胞培养/骨保护因子Key words
Human periodontal ligament cells/Recombinant human interleukin-1β/Cell culture/Osteoprotegerin分类
生物科学引用本文复制引用
张侬,谢妮,付云,舒睿,杨雅..人白介素－1β水平与人牙周膜细胞OPG表达关系[J].临床口腔医学杂志,2014,(12):716-717,718,3.
