临床口腔医学杂志Issue(1)：20-23,24,5.DOI:10.3969/j.issn.1003-1634.2015.01.008
多西环素对大鼠正畸移动牙根吸收影响的研究
An experimental study on root resorption tissue during orthodontic tooth movement in rats with doxycycline administration
张志菲 1刘月华2
作者信息
- 1. 上海交通大学附属瑞金医院卢湾分院口腔科 上海 200020
- 2. 同济大学口腔医学院口腔正畸科 上海 200072
- 折叠
摘要
Abstract
Objective:To investigate the root-resorbing tissue during experimental tooth movement with doxycycline (DC) administration,to elucidate the protein changes and mechanism of root resorption induced by orthodontic tooth move-ment. All of these could give an evidence which maybe used regulate orthodontic tooth movement more efficiently. Method:Animal models of experimental orthodontic tooth movement in Sprague-Dawley rats were established. 36 male rats were ran-domly assigned to four groups. Nine animals served as untreated controls. Nine animals were only administered DC for 7 days,by means of injection on rat's back subcutaneously daily. In 18 animals the maxillary first molar was moved mesially by a continuous and constant reciprocal force of 50 g from Sentalloy®closed-coil springs. In 9 of these animals DC was ad-ministered at the time of appliance insertion and throughout the experiment. The animals were sacrificed 7,10 or 14 days af-ter force application and block sections processed for Haematoxylin and eosin(HE) staining and tartrate resistant acid phos-phatase (TRAP). The root resorption area and the number of TRAP-postive cells on root were counted at the compressing side of the mesial roots. Computer image analysis was performed to evaluate all of these results. Result:①The animals in the doxy-ortho group had significantly less root resorption than the animals in the ortho-group at all experimental periods,except day 14. In the doxy-ortho group, the mean of root resorption increased throughout the experiment,while the ortho group re-vealed a reduction in the mean of root resorption by day 14.②In both groups the number of TRAP-positive cells presented a peak at day 10 and decreased towards day 14. The doxy-ortho group demonstrated significantly fewer TRAP-positive cells on the root surface than the ortho group after 10 days of force application. Conclusion:DC may have an inhibitory effect on the recruitment of TRAP-positive cells and orthodontically induced resorptive activity.关键词
牙根吸收/多西环素/TRAP阳性细胞/正畸牙齿移动Key words
root resorption/doxycycline/orthodontic tooth movement/TRAP-positive cells分类
医药卫生引用本文复制引用
张志菲,刘月华..多西环素对大鼠正畸移动牙根吸收影响的研究[J].临床口腔医学杂志,2015,(1):20-23,24,5.
