临床口腔医学杂志Issue(3)：131-133,3.DOI:10.3969/j.issn.1003-1634.2015.03.002
BMSCs复合PRF修复牙槽骨缺损中OPG和RANKL的表达及意义
Expression and significance of OPG and RANKL protein in repairing alveolar bone defect with bone marrow stem cells(BMSCs) compounded with platelet-rich fibrin(PRF)
摘要
Abstract
Objective:To observe expression and significance of osteoprotegerin(OPG) and receptor activator of NF kappa B ligand(RANKL) protein in alveolar bone repair with bone marrow stem cells(BMSCs) compounded with platelet-rich fibrin(PRF) in rabbits. Method:Thirty-six male 2 months rabbits were divided into four groups(A,B,C,D) randomly. A group:BMSCs/PRF composite;B group:PRF;C group:BMSCs;D group:blank control.They were minimally invasive re-moval mandible incisor under general anesthesia on the left side.The rabbits were sacarificed at 3 time point(4,8,12 week) with nine sacarificed per time postoperatively and then the jaws were taken out immediately. Immumohistochemistry was performed at each of 3 time points respectively to detect the expression of OPG and RANKL. Result:OPG protein expres-sion in group A,B,C was significantly higher than blank control at 4,8,12 week (P <0.05) and expression of RANKL in group A,B,C was significantly higher than blank control at 4,8,12 week (P<0.05);the expression of OPG and RANKL in BMSCs/PRF composite were higher than using BMSCs or PRF alone in factorial analysis (P <0.05). Conclusion:BMSCs composite PRF can increase the expression of OPG, RANKL in repairing alveolar bone defect and could help alveolar bone reconstruction.关键词
骨保护素/核因子B 受体活化因子配体/骨髓间充质干细胞/富血小板纤维蛋白Key words
OPG/RANKL/bone marrow stem cells/platelet-rich fibrin分类
医药卫生引用本文复制引用
张文丽,陈诚,李淑慧,李一鸣,周晶,吴佩玲..BMSCs复合PRF修复牙槽骨缺损中OPG和RANKL的表达及意义[J].临床口腔医学杂志,2015,(3):131-133,3.基金项目
新疆维吾尔自治区青年科学基金项目 （）
