临床口腔医学杂志Issue(3)：148-151,4.DOI:10.3969/j.issn.1003-1634.2015.03.007
不同细胞外基质对人胚胎干细胞体外定向诱导分化为角化细胞的影响
Influence of different extracellular matrix to induced differentiation of Human Embryonic Stem Cells to Ker-atinocytes in vitro
摘要
Abstract
Objective:To research different extracellular matrix's influence to kerotinocyte derived from Human Em-bryonic Stem Cells about growth,proliferation and differentiation. Method:Culture the human embryonic stem cells In vitro and induced by RA、BMP4、AA,then culture the cells in the dishs coated with Matrigel (A)、CollagenⅠ(B)、CollagenⅣ(C)、Gelatin(D),and nothing coating the dish(E). TO observe the cell growth by inverted microscope,Detect cell prolifer-ation With CCK8,The expression of CK14 is detected by the western-blotting. Result:The E-KCs grow faster and have the stronger proliferation abilities than other groups. Cell colony formation rate is high and T test shows P <0.05 compared with other groups. Western-blotting shows that the expression of CK14 of A group is higher than other groups ,P<0.01,the differ-ence is statistically significant. Conclusion:Matrigel can significantly improve the ability of human embryonic stem cells in-ducing into the keratinocytes, and can promote the proliferation of E-KCs.关键词
胚胎干细胞/细胞外基质/诱导分化/角化细胞Key words
embryonic stem cells/extracellular matrix/induced differentiation/keratinocytes分类
生物科学引用本文复制引用
赵厚明,李晗卿,周海文..不同细胞外基质对人胚胎干细胞体外定向诱导分化为角化细胞的影响[J].临床口腔医学杂志,2015,(3):148-151,4.基金项目
国家自然科学基金项目资助（81170968）；2013～2014年度国家临床重点专科建设项目资助 （）
