临床口腔医学杂志Issue(4)：206-208,209,4.DOI:10.3969/j.issn.1003-1634.2015.04.005
吸烟对口腔黏膜上皮细胞NOD1信号通路关键分子表达水平的影响
Effects of cigarette smoke on key molecules of NOD1 signaling in human oral mucosal epithelial cells
摘要
Abstract
Objective:To evaluate the effect of Cigarette Smoke on Key Molecules of NOD1 Signaling in human oral mucosal epithelial cells. Method:The cells were stimulated using a smoke chamber with cigarette smoke for different times (5、10、20、40、60 min) in certain experiments. The control group cells were cultured with 5 % CO2 for 10 min. The crucial protein expression in NOD1 Signaling was measured by Immunofluorescence assays. Result: Cigarette Smoke down-regu-lated NOD1 protein levels and up-regulated Caspase 12 and RIP2 protein in a time-dependent manner. The NF-κB protein level reached the peak level after stimulated for 10 mins. Conclusion:Cigarette smoke could play a inhibitory effects on NOD1 Signaling even innate immunity in human oral mucosal epithelial cells.关键词
NOD1/吸烟/固有免疫Key words
NOD1 Signaling/Cigarette smoke/innate immunity分类
医药卫生引用本文复制引用
高雅凡,王文梅,王翔,叶沛,钱雅洁,黎景景..吸烟对口腔黏膜上皮细胞NOD1信号通路关键分子表达水平的影响[J].临床口腔医学杂志,2015,(4):206-208,209,4.基金项目
国家自然科学基金项目（81070839）；江苏省临床医学科技专项（BL2014018）；南京市科技计划项目 （）
