临床口腔医学杂志2015，Vol.31Issue(5)：263-265,3.DOI:10.3969/j.issn.1003-1634.2015.05.003
尼古丁对大鼠牙槽骨内ALP和DMP1 C末端表达的影响
The effects of nicotine on the expression of ALP and the DMP1 C terminal in rat alveolar bone
摘要
Abstract
Objective:To observe the effects of nicotine on the expression of Alkaline Phosphatase (ALP)and the dentin matrix protein (DMP)1 C terminal in rat alveolar bone. Method:Twenty health male Wistar rats of five weeks of age were randomly divided into experimental group and control group,each group was randomly divided into two subgroups:30 and 60 days,the experimental group was to nicotine 0.73 mg·kg-1·d-1 by intraperitoneal injection. The expression of ALP and the DMP1 C terminal in the alveolar bone was respectively detected by calcium cobalt method and immun-ohistochem-istry. Result:Control group:ALP and DMP1 C terminal linear expressed in the calcification front of the alveolar boneand it is dark brown,apparent mineralization strap was formed;ALP brown precipitation and DMP1 C term-inal brown yellow gran-ular dense clouded around the bone cells;Experimental group:ALP and DMP1 C terminal parts of the same expression in the control group,decreased intensity of expression,the change of 60 days is more obvious than 30 days. Conclusion:Nico-tine may inhibit mineralization of rat alveolar bone by declining the expression of ALP and DMP1 C terminal in rat alveolar bone.关键词
尼古丁/牙槽骨/碱性磷酸酶/牙本质基质蛋白1Key words
Nicotine/Alveolar bone/Alkaline phosphatase/Dentin matrix protein 1分类
生物科学引用本文复制引用
邱萌,马肃,侯琳,张宇,陈力,秦春林..尼古丁对大鼠牙槽骨内ALP和DMP1 C末端表达的影响[J].临床口腔医学杂志,2015,31(5):263-265,3.基金项目
黑龙江省科技厅自然科学基金资助(D2004-43) （D2004-43）
黑龙江省科技厅科研基金资助(2010-007) （2010-007）
