临床神经病学杂志Issue(2)：121-124,4.
缺血预处理对局灶性脑缺血再灌注大鼠缺血侧海马CA1区低氧诱导因子－1α及存活素表达的影响
Effects of ischemic preconditioning on the expression of hypoxia-inducible factor-1αand survivin at ischemia bilateral hippocampal CA1 area in rats with focal cerebral ischemia reperfusion
摘要
Abstract
Objective To observe the effects of ischemic preconditioning on the expression of hypoxia-inducible factor-1α( HIF-1α) and survivin at ischemia bilateral hippocampal CA1 area in rats with focal cerebral ischemia reperfusion.Methods One hundred and thirty healthy male SD rats were randomly divided into sham operation group ( SO group, n =10 ) , focal cerebral ischemia reperfusion group ( MCAO group, n =60 ) and ischemia preconditioning group ( BIP group, n=60) .The MCAO group and BIP group were further divided into reperfusion 2 h, 6 h, 12 h, 24 h, 48 h and 72 h 6 subgroups, respectively, with 10 rats in each subgroup.Sutured method was used to made focal cerebral ischemia reperfusion model rats.The BIP group received ischemic preconditioning with 10 min at 24 h before cerebral ischemia.The numbers of positive cells of HIF-1αand survivin were detected by immunohistochemistry.The relative contents of protein of HIF-1αand survivin were detected by Western blot method.Results Compared with the SO group, the numbers of positive cells and the relative contents of protein of HIF-1α, survivin at ischemia bilateral hippocampal CA1 area in subgroups at each time point of MCAO group and BIP group were significantly increased ( all P<0.05 ) .Compared with the MCAO group, the numbers of positive cells and the relative contents of protein of HIF-1α, survivin at ischemia bilateral hippocampal CA1 area in subgroups at each time point of BIP group were significantly increased ( all P<0.05) .In the MCAO group and BIP group, the numbers of positive cells and the relative contents of protein of HIF-1α, survivin at ischemia bilateral hippocampal CA1 area were all arrived the peak at reperfusion 24 h moment ( all P<0.05 ) .Conclusions Ischemic preconditioning can increase the expression of HIF-1α, survivin at ischemia bilateral hippocampal CA1 area in rats with focal cerebral ischemia reperfusion.It may be related to the mechanism of cerebral ischemia tolerance.关键词
缺血预处理/脑缺血再灌注/低氧诱导因子-1α/存活素/脑缺血耐受Key words
ischemic preconditioning/cerebral ischemia reperfusion/hypoxia-inducible factor-1α/survi-vin/cerebral ischemia tolerance分类
医药卫生引用本文复制引用
王红霞,李世英,李峥,张晋霞,贺永贵,余红,孙妍,刘斌..缺血预处理对局灶性脑缺血再灌注大鼠缺血侧海马CA1区低氧诱导因子－1α及存活素表达的影响[J].临床神经病学杂志,2015,(2):121-124,4.基金项目
河北省2013年医学科学研究重点课题计划 （）
