临床神经病学杂志Issue(3)：205-209,5.
匹罗卡品致癫痫小鼠海马中精神分裂症断裂基因1蛋白的表达及其在癫痫中的作用
Expression of disrupted-in-schizophrenia 1 in the hippocampus of epileptic mice induced by pilocarpine and its effect on epilepsy
摘要
Abstract
Objective To studied the protein and mRNA level of the disrupted-in-schizophrenia 1 ( DISC1 ) in the hippocampus of epileptic mice induced by pilocarpine and its effect on epilepsy .Methods Two hundreds and six male C57BL/6 mice were randomly diveded into experimental group (n=198) and control group (n=48),the mice in experimental group were given small dosage of pilocarpine to set up the epileptic mice model .Expression of DISC1 and minichromosome maintenance protein 2 ( MCM2 ) in the hippocampus of two groups were analysed via immunohistochemistry and real-time PCR at different time points (3,7,14,28 d) after status epilepticus.Results Compared with the control group ,the quantity of MCM2 posotive cells and MCM2 mRNA in experimental group were significant increased at each time point (P<0.05-0.01), which appeared a decreasing tendency in control group (all P<0.05).Compared with the control group, the expression level of DISC1 and DISC1 mRNA at dentate gyrus and CA3 area in experimental group were significantly lower from 7 d(P<0.05-0.01).A significant correlation existed between the expression level of DISC 1 and MCM2 according to Pearson correlation analysis ( P<0.01 ) .Conclusion DISC1 may participate in the pathogenesis of epilepsy by mediating the proliferation of new neurons .关键词
精神分裂症断裂基因1/新生神经元/癫痫Key words
disrupted-in-schizophrenia 1/new neurons/epilepsy分类
医药卫生引用本文复制引用
方红军,廖红梅,杨赛,冯枚,胡文静,王平,陈玫,肖波..匹罗卡品致癫痫小鼠海马中精神分裂症断裂基因1蛋白的表达及其在癫痫中的作用[J].临床神经病学杂志,2015,(3):205-209,5.基金项目
国家自然科学基金资助项目 （）
