临床神经外科杂志Issue(3)：161-164,4.DOI:10.3969/j.issn.1672-7770.2014.03.001
吴茱萸碱对人胶质瘤 U251细胞增殖及凋亡影响的研究
Effects of evodiamine on proliferation and apoptosis of human glioma cell U251
摘要
Abstract
Objective To study the effect of evodiamine on human glioma cell U 251 and explore the mechanism .Methods Human glioma cells U251 were in vitro cultured .They were divided into the control group and 25, 50 and 100 μg/mL evodiamine groups .The proliferation rate was detected at 24, 48, and 72 h using MTT assay .Hoechst 33258 staining was used to visualize nuclear changes and apoptotic body formation .The early apoptosis rate at 24 h was detected by flow cytometry .The expressions of apoptosis-related proteins were detected at 48 h respectively using western blot analysis . Results Compared with the control group , the growth inhibition rate were significantly increased in all evodiamine groups at 24, 48, and 72 h.Compared to the control cells , cells exposed to evodiamine presented typical apoptotic morphology and formation of apoptotic bodies .The early apoptosis rate was 8.65%, 19.47%, and 28.97%in 25, 50 and 100μg/mL evodiamine groups , respectively, showing statistical difference when compared with the control group .Western blotting analysis showed that the expression of apoptosis related proteins FAS , FADD, Caspase-8 and Caspase-3 were significantly upregulated , Bcl-2 was downregulated and Bax was upregulated .All the above experiments were in time-and dose-dependent manners .Conclusions Evodiamine significantly inhibited the proliferation and promoted the apoptosis of glioma cancer cell U 251 in a dose-and time-dependent manner .The mechanism may be related to the upregulation of Fas signaling pathways and downregulation of Bcl -2/Bax .关键词
吴茱萸碱/胶质瘤/增殖/凋亡Key words
evodiamine/glioma/proliferation/apoptosis分类
医药卫生引用本文复制引用
曹志,杨伊林,王穗暖,屈洪涛,彭亚,王榕..吴茱萸碱对人胶质瘤 U251细胞增殖及凋亡影响的研究[J].临床神经外科杂志,2014,(3):161-164,4.基金项目
国家自然科学基金资助项目 （）
