临床神经外科杂志Issue(3)：172-174,177,4.DOI:10.3969/j.issn.1672-7770.2014.03.004
E2F1蛋白在胶质瘤中的表达及其对胶质瘤细胞生长的影响
Expression of E2F1 protein in glioma and its effect on growth of human glioma cell
王炜 1尤永平2
作者信息
- 1. 210029 南京医科大学第一附属医院神经外科
- 2. 安徽省铜陵市人民医院神经外科
- 折叠
摘要
Abstract
Objective To study the expression of E 2 F1 protein in human glioma tissues and its effect on tumor growth .Methods E2F1 expression in glioma tissues was determined by real-time quantification RT-PCR.Subsequently, MTT assay, western blotting and Glucose Assay were used to assay U87 cells with reduced E2F1 expression.Results This data of the study showed that the expression of E2F1 in glioma was increased and strongly correlated with its pathological grade . Abrogating expression of E2F1 could suppress glioma cells growth , decrease the glucose consumption.Conclusions The expression levels of E2F1 mRNA in human glioma is high. Abrogating expression of E 2F1 could inhibit the growth and metabolism in U 87 cells.关键词
胶质瘤/E2F1蛋白/增殖/肿瘤代谢Key words
glioma/E2 F1/proliferation/cancer metabolism分类
医药卫生引用本文复制引用
王炜,尤永平..E2F1蛋白在胶质瘤中的表达及其对胶质瘤细胞生长的影响[J].临床神经外科杂志,2014,(3):172-174,177,4.
