临床神经外科杂志Issue(5)：326-329,334,5.DOI:10.3969/j.issn.1672-7770.2014.05.002
PEX 基因对 C6胶质瘤干细胞增殖作用影响的研究
Study on effect of PEX gene-modified C6 glioma stem cells on proliferation
摘要
Abstract
Objective To study the effects of PEX gene on proliferation and cell cycle of C 6 glioma stem cells in vitro .Methods Using traditional serum medium cultured C 6 cell lines and taking the single-celled levitation liquid suspension subculture in serum-free, brain tumor stem cells were separated and identified by immune fluorescence staining with pDsRed 2-C1-PEX which the lab successful constuction were transfected .In the vitro brain tumor stem cells ( BTSCs ) by liposome-mediated,and then was detected by using reverse transcriptase-polymerase chain reaction .Effects of PEX on BTSCs growth were observed by water soluble tetrazolium cell proliferation and cytotoxicity assay kit .The cell cycle changes of BTSCs was detected by flow cytometry analysis .Results The BTSCs were cultured successfully and were transfected by PEX high expression of PEX in BTSCs in vitro and its inhibition of the proliferation of BTSCs were detected .The group which carrying PEX cell cycle G0/G1 phase cell percentage rised to 81 .10%, S period cells decreased to 12 .04%, statistical disclosed significance ( P <0.05 ).Conclusion PEX gene in C6 glioma, and its expression of the stem cells in vitro can directly suppress the BTSCs proliferation .关键词
PEX基因/胶质瘤干细胞/真核表达载体Key words
PEX gene/glioma stem cells/eukaryotic expression vector分类
医药卫生引用本文复制引用
黄炜,张云峰,高歌..PEX 基因对 C6胶质瘤干细胞增殖作用影响的研究[J].临床神经外科杂志,2014,(5):326-329,334,5.基金项目
国家自然科学基金项目 （）
