临床神经外科杂志Issue(5)：335-338,343,5.DOI:10.3969/j.issn.1672-7770.2014.05.004
uPAR 促进恶性脑膜瘤细胞的侵袭
uPAR promotes malignant meningioma cells invasion
李正阳 1吾太华 1郑传红 2邓少勇 1肖贤军 1苏世星 1马赞 1左徳献1
作者信息
- 1. 510510 广州，广东三九脑科医院神经外科
- 2. 河南省南阳市中心医院急诊科
- 折叠
摘要
Abstract
Objective To explore the relationships among tumor grade and levels of urokinase-type plasminogen activator(uPA) or its receptor(uPAR) in meningiomas.The function of uPAR and its molecular mechanisms in malignant meningiomas were also studied .Methods We detected the protein levels of uPAR and uPA in 58 samples of meningiomas to analyze whether the protein levels of uPAR/uPA were related to tumor grade of meningiomas .The expression of uPAR to study whether uPAR affects the invasion of malignant meningioma cells and the expression levels of invasive molecules ( MMP-2 , MMP-9 and E-cadherin ) were knocked down .Results The protein levels of uPAR and uPA was proportional to malignant degree of meningiomas .In normal brain tissues ,uPAR and uPA were not detected , in grade Ⅰ meningiomas , uPAR and uPA were mainly weekly stained ( week:uPA 73 .30%, uPAR 66 .66%) .In grade Ⅱ meningiomas , uPAR and uPA were equably stained in each grade meningiomas ( week:uPA 37.50%,uPAR 18.75%,moderate:uPA 37.50%, uPAR 43.75%;strong:uPA 25.00%,uPAR 37.50%).In grade Ⅲ meningiomas,uPAR and uPA were mainly strongly stained ( strong:uPA 66.6%,uPAR 83.33%).Knockdown of uPAR did not affect the expression of uPA , but strongly decreased the levels of MMP-2 and MMP-9 .Knockdown of uPAR also decreased the invasion of malignant meningioma cells .Conclusion The uPA-uPAR system was extensively expressed in meningiomas , and its expression level was proportional to malignant degree of meningiomas .uPAR shows ability to increase the invasion of malignant meningioma cell by regulating MMP2 and MMP9.关键词
脑膜瘤/侵袭/尿激酶型纤溶酶原激活物/基质金属蛋白酶Key words
meningioma/invasion/uPAR/MMPs分类
医药卫生引用本文复制引用
李正阳,吾太华,郑传红,邓少勇,肖贤军,苏世星,马赞,左徳献..uPAR 促进恶性脑膜瘤细胞的侵袭[J].临床神经外科杂志,2014,(5):335-338,343,5.
