临床神经外科杂志Issue(1)：43-47,5.DOI:10.3969/j.issn.1672-7770.2015.01.012
脓毒症大鼠模型脑胰岛素生长因子1表达的变化及影响
Changes of insulin-like growth factor-1 expression and its effects on brain of rat sepsis model
杨阳 1王强 1满明昊 1李玉骞 1李立宏1
作者信息
- 1. 710038西安，第四军医大学唐都医院神经外科
- 折叠
摘要
Abstract
Objective To explore the change of insulin-like growth factor-1( IGF-1) expression and its effects on cerebral cortex of rat sepsis model .Methods The rat model of sepsis was established by cecal ligation and puncture ( CLP ) .The IGF-1 positive cells were observed by immunofluorescent staining .IGF-1 and caspase-3 expression was determined by Western blot . TUNEL staining was used to assess neuron apoptosis .Then septic rats were treated with IGF-1 or saline by lateral ventricle injection .The expression of caspase-3 was evaluated by western blot . Neuron apoptosis was assessed by TUNEL staining .Results Compared with the sham-operated group, IGF-1 positive cells in cerebral cortex in the sepsis group decreased obviously .Besides, IGF-1 expression decreased , caspase-3 expression and neuron apoptosis increased (all P<0.05). When IGF-1 was given , caspase-3 expression and cell apoptosis was similar to the sham-operated group, while in the saline-treated group, the results were similar to the sepsis group .Conclusion During sepsis , IGF-1 expression in rat cerebral cortex obviously decreased and neuron apoptosis increased.After treated with exogenous IGF-1, cell apoptosis decreased .These findings suggested that IGF-1 may protect rat brain from sepsis by inhibiting the up-regulation of caspase-3.关键词
脓毒症/凋亡/胰岛素生长因子1/大鼠模型Key words
sepsis/cecal ligation and puncture/apoptosis/insulin-like growth factor-1/rat model分类
医药卫生引用本文复制引用
杨阳,王强,满明昊,李玉骞,李立宏..脓毒症大鼠模型脑胰岛素生长因子1表达的变化及影响[J].临床神经外科杂志,2015,(1):43-47,5.
