临床神经外科杂志Issue(2)：107-110,4.DOI:10.3969/j.issn.1672-7770.2015.02.008
丁苯酞对大鼠脑创伤后 caspase-3的表达与细胞凋亡的影响
宋朝彦 1董晓辉 1谢东1
作者信息
- 1. 071000 保定市第一中心医院神经外科
- 折叠
摘要
Abstract
Objective To explore the casepase-3 expression and role of casepase-3 in apoptosis following traumatic brain injury(TBI) in rat and the effect of dl-3-n-butylphthalide(NBP).Methods 120 male Wistar rats were randomly divided into 3 groups .According to Marmarou , severe closed brain injury was made .After that , immunohistochemical staining with Caspase-3 were performed of hippocampus CA1 region.TUNEL in situ was applied of the apoptosis cell in hippocampus CA 1 region.Double staining was to show the relation of apoptosis and Caspase -3.Results Caspase-3 in the trauma group began to increase at 3 h following injury , peaked at 3rd d and began to decline at 7 d. Caspase-3 in NBP treatment group was the same in each time , but in the peak , Caspase-3 was decreased in NBP treatment group compared with that in trauma group (P<0.01).At 3 h in trauma group , a little TUNEL positive cell appeared in hippocampus , peaked at 3rd d and began to decline at 7 d.TUNEL positive cell in NBP treatment group was the same in each time ,but in the peak , TUNEL positive cell was decreased in NBP treatment group compared with that in trauma group (P<0.05). The expression of casepase-3 was correlated with TUNEL positive cells ( P<0.01) .Conclusion NBP treatment in rats after TBI has certain neuroprotective effect , whose resistance roles in nerve cell apoptosis may be implemented by inhibiting excessive expressed caspase -3 caused by TBI .关键词
大鼠/颅脑创伤/casepase-3/凋亡/丁苯酞Key words
rats/traumatic brain injury/caspase-3/apoptosis/dl-3-n-butylphthalide分类
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宋朝彦,董晓辉,谢东..丁苯酞对大鼠脑创伤后 caspase-3的表达与细胞凋亡的影响[J].临床神经外科杂志,2015,(2):107-110,4.
