临床误诊误治Issue(6)：100-104,5.DOI:10.3969/j.issn.1002-3429.2014.06.039
重组人碱性成纤维细胞生长因子对兔视网膜缺血再灌注损伤的保护作用
Protective Effect of Recombinant Human Basic Fibroblast Growth Factor on Retinal Ischemia-reperfusion Injury in Rabbits
摘要
Abstract
Objective To explore the protective effect of recombinant human basic fibroblast growth factor (rh-bFGF) on retinal ischemia-reperfusion injury (RIRI) in rabbits. Methods The 66 healthy purebred rabbits were randomly divided into sham-operation group (n=6), ischemia-reperfusion model group (n=30) and rh-bFGF treatment group (n=30). The RIRI models were established by elevating intraocular pressure, the ischemia-reperfusion model group was injected with 0. 9% sodium chloride injec-tion;rh-bFGF drug therapy was performed in rh-bFGF treatment group;the sham-operation group only received the 5th scalp heedle from ear piercing the anterior chamber angle in front of the sclera edge, and then the needle was slowly removed. Retina histopatho-logical changes were observed by routine hematoxylin and eosin stain 6 h, 12 h, 24 h, 48 h and 72 h after RIRI in ischemia-reperfu-sion model and rh-bFGF treatment groups. Expressions of inhibiting gene Bcl-2 and stimulative gene Bax and changes of Bax/Bcl-2 in retinal tissues were detected with SABC immunohistochemical method. Results No apoptotic cell was found in sham-operation group;apoptotic cells mainly existed in the inner nuclear layer and the ganglion cell layer, and apoptotic cells reached the peak 24 h after reperfusion;the rh-bFGF treatment groups had the similar condition with the ischemia-reperfusion model group in cell in-jury at each observational time. No expressions of Bcl-2 and Bax positive cells were found in normal retina tissues;the expressions were found 6 h after reperfusion, and the expressions reached the peak 24 h after reperfusion, and began to descented 48 h after reperfusion, and the decrease was obvious 72 h after reperfusion. Bcl-2 expression was significantly stronger, while Bax expression was significantly reduced in rh-bFGF treatment group, compared with those in ischemia-reperfusion model group (P<0. 05). Con-clusion Bcl-2 and Bax protein are involved in the RIRI regulation. The retina tissue can be protected with Bcl-2 expression of up-regulation and the Bax expression of down-regulation by rh-bFGF, which can increase the Bcl-2 /Bax ratio.关键词
重组人碱性成纤维细胞生长因子/视网膜/再灌注损伤/Bcl-2/BaxKey words
Recombinant human basic fibroblast growth factor/Retina/Reperfusion injury/Bcl-2/Bax分类
医药卫生引用本文复制引用
邵宏超,葛嫣然,李利艳,王林洪,马建英,曹凤英,田华..重组人碱性成纤维细胞生长因子对兔视网膜缺血再灌注损伤的保护作用[J].临床误诊误治,2014,(6):100-104,5.基金项目
唐山市科技计划项目(12140209A-45) （12140209A-45）
