临床误诊误治Issue(6)：111-115,5.DOI:10.3969/j.issn.1002-3429.2014.06.042
针刺对过敏性哮喘大鼠肺肠组织SP-A mRNA表达的影响
Influence of Acupuncture on the SP-A mRNA Expression in Lung and Intestine Tissues of Rats with Allergic Asthma
摘要
Abstract
Objective To explore the influence of acupuncture on the expression of pulmonary surfactant protein (SP-A) mRNA in the lung and intestine tissues of rats with allergic asthma. Methods A total of 40 SD rats were randomly divided into control group (n=8), allergic asthma model group (n=8), the lung acupuncture group (n=8), the intestine acupuncture group (n=8) and the lung and intestine acupuncture group (n=8). Except in the control group, rats in the rest four groups re-ceived egg album by intraperitoneal injection to induce allergy, and the models of allergic asthma were established by egg albu-min of intravenous injection in vena caudalis. Rats in control and allergic asthma model groups were not treated. Rats in lung ac-upuncture group received acupuncture treatment in acu-points of Chize ( LU11 ) , Kongzui ( LU6 ) , Lieque ( LU7 ) and Feishu (BL13);rats in intestine acupuncture group received acupuncture treatment in acu-points of Quchi (LI11), Hegu (LI4), Tian-shu (ST25) and Shangjuxu (ST37); rats in the lung and intestine acupuncture group received acupuncture treatment in acu-points of Kongzui (LU6), Lieque (LU7), Quchi (LI11) and Tianshu (ST25). The SP-A mRNA expressions were detected and compared in the five groups after the treatment. Results The expressions of SP-A mRNA in the tissues of lung, large intestine and small intestine in the allergic asthma model groups were significant higher than those in control group (P<0. 01);the ex-pressions of SP-A mRNA in the tissues of lung and large intestine in the lung acupuncture, the intestine acupuncture and the lung and intestine acupuncture groups and small intestine tissues in the lung and intestine acupuncture group were significantly higher than those in the allergic asthma model group (P<0. 05);the differences in expressions of SP-A mRNA in small intes-tine tissues between the lung acupuncture and the intestine acupuncture groups with allergic asthma model group showed no sta-tistical significances (P>0. 05). Conclusion The SP-A mRNA expressions in the lung and intestine tissues of rats with aller-gic asthma are of bnormality, and the lung has significant biological relationship with the large intestine.关键词
肺/肠/哮喘/大鼠/针刺/膜蛋白质类/RNA,信使Key words
Lung/Intestine/Asthma/Rat/Acupuncture/Membrane proteins/RNA, messenger分类
医药卫生引用本文复制引用
王宝凯,付钰,袁之林,杨超,王霜,赵吉平..针刺对过敏性哮喘大鼠肺肠组织SP-A mRNA表达的影响[J].临床误诊误治,2014,(6):111-115,5.基金项目
国家重点基础研究发展计划(973计划)资助项目(2009CB522708) （973计划）
