临床误诊误治Issue(12)：102-105,4.DOI:10.3969/j.issn.1002-3429.2014.12.040
蜂毒多肽对人膀胱癌T24细胞浸润能力的影响
Effect of Venom Peptides on the Invasion of T24 Cells in Human Bladder Cancer
王强 1张志杰 2祁宏伟 2谢亚彬 2杨桂云 3韩春伟 4牛勇5
作者信息
- 1. 071000河北保定，解放军252医院泌尿外科
- 2. 071051河北保定，保定市第二医院麻醉科，药剂科，泌尿外科
- 3. 071000河北保定，保定市第一中心医院体检科
- 4. 071000河北保定，保定市第三中心医院口腔科
- 5. 073100 河北 曲阳，曲阳县人民医院普外科
- 折叠
摘要
Abstract
Objective To investigate the effect of venom peptides on the invasion of human bladder carcinoma cell line T24. Methods Various concentrations of venom peptides (0, 0. 1, 1. 0, 10. 0, 100. 0 mg/ml) were acted on to human bladder cancer cell line T24 ( control group and venom peptides group in different concentrations) in order to examine the ad-hesion,infiltration and migration ability of human bladder cancer cell line T24 by MTT assay, Transwell assay and scratch test respectively. Results Adhesion rate of various concentrations of venom peptides group was significantly lower than that of control group (P<0. 05);while the percentage of venom peptides increased, adhesion rate decreased progressively, and there was significant difference between the two groups in various concentrations of venom peptides groups (P<0. 05). Cell popula-tion penetrating the membrane in the 24 hour culture of various concentrations of venom peptides group was significantly de-creased, compared with that in control group ( P<0. 05 ); Cell population penetrating the membrane decreased progressively in the wake of the concentration of venom peptides increasing, and there was significant difference between the two groups in various concentrations of venom peptides group (P<0. 05). 12 and 24 hours after scratching, cell non-coverage area of vari-ous concentrations of venom peptides group increased, compared with that of control group at the same time, and there was significant difference between the two groups in various concentrations of venom peptides group (P<0. 05);Cell non-coverage area increased progressively along with the concentration of venom peptides increasing, and there was significant difference be-tween the two groups at the same time in various concentrations of venom peptides group ( P<0. 05 ) . Conclusion Venom peptide possesses an inhibitory effect against the adhesion,infiltration and migration ability of human bladder cancer cell line T24, which increases along with the increasing of concentrations of venom peptides in a dose-dependent manner.关键词
体外实验/蜂毒多肽/膀胱肿瘤/浸润Key words
In vitro experiment/Venom peptide/Urinary bladder neoplasm/Infiltration分类
医药卫生引用本文复制引用
王强,张志杰,祁宏伟,谢亚彬,杨桂云,韩春伟,牛勇..蜂毒多肽对人膀胱癌T24细胞浸润能力的影响[J].临床误诊误治,2014,(12):102-105,4.
