临床误诊误治Issue(4)：108-112,5.DOI:10.3969/j.issn.1002-3429.2015.04.036
冬凌草甲素对急性单核细胞白血病细胞株THP-1的作用研究
Anti-leukemia Effect of Oridonin on Acute Monoblastic Leukemia
摘要
Abstract
Objective To investigate the anti-leukemia effect of oridonin on acute monoblastic leukemia cell line THP-1. Methods Acute monoblastic leukemia cell line THP-1 was cultured in vitro. Cell proliferation Inhibition rate after 72 h of treatment of 4, 6, 8 μmol/L oridonin was examined using modified MTT assay, cell survival rate after 24, 72, 120 h of treatment of 2, 4, 6, 8,10 μmol/L oridonin was calculated and the growth curve was drawn. The cellular morphologic changes after 24 h of treatment of 4, 6, 8μmol/L oridonin were observed under a light microscope. The percentage of apopto-sis of THP-1 cells after 24 h of treatment of 0, 4, 6, 8μmol/L oridonin was evaluated using flow cytometric analysis. The ac-tivation levels of Akt/mTOR, Raf/MEK/ERK signaling pathways and the expression levels of Bcl-2 and Bax after 0, 12, 24 h of treatment of 6 μmol/L oridonin were examined by Western blot. Results ① Cell proliferation inhibition rate after 72 h of treatment of 4, 6, 8 μmol/L oridonin was (20. 02 ± 11. 15)%, (45. 99 ± 12. 76)%, (86. 39 ± 9. 18)% respectively, com-pared with 4 μmol/L oridonin treatment group, cell proliferation inhibition rate after 6, 8 μmol/L oridonin treatment were higher, with statistically significant differences (P<0. 05 or P<0. 01). Cell growth curve showed that oridonin inhibited the growth of THP-1 cells in time-and dose-dependent manner and the IC50 of oridonin was (6. 12 ± 1. 48)μmol/L after 72 h of treatment. ② After 24 h of treatment of oridonin, THP-1 cells apoptosis occurred and apoptotic bodies were formed, the high-er the concentration of oridonin, the more apoptotic bodies were. ③The percentage of apoptosis rate after 24 h of treatment of 0, 4, 6, 8 μmol/L oridonin were (3. 7 ± 1. 1)%, (16. 2 ± 3. 3)%, (30. 1 ± 4. 3)% and (49. 5 ± 6. 7)% respectively. The differences of apoptosis rate between various oridonin concentration groups and control group were statistically significant (P<0. 05 or P<0. 01). The differences between various oridonin concentration groups were also statistically significant (P<0. 05). ④ 6 μmol/L oridonin treatment inhibited the activation of Akt/mTOR and Raf/MEK/ERK signaling pathways in THP-1 cells, and down-regulated the level of anti-apoptotic protein Bcl-2 and up-regulated the expressions of pro-apoptotic protein Bax. Conclusion Oridonin exerts anti-leukemia effect in THP-1 cells by inhibiting the activation of Akt/mTOR and Raf/MEK/ERK signaling pathways, regulating the expression of Bcl-2 and Bax apoptosis-modulating proteins.关键词
冬凌草素/白血病,单核细胞,急性/白血病抑制因子/凋亡调节蛋白质类Key words
Oridonin/Leukemia, monocyte, acute/Leukemia inhibitory factor/Apoptosis-modulating protein分类
医药卫生引用本文复制引用
郭勇,单卿卿,龚玉萍,林娟,杨曦..冬凌草甲素对急性单核细胞白血病细胞株THP-1的作用研究[J].临床误诊误治,2015,(4):108-112,5.基金项目
国家自然科学基金(30770912) （30770912）
四川省科技厅社会公益项目(2008 SZ0017) （2008 SZ0017）
教育部回国留学人员启动金(20071108-18-4) （20071108-18-4）
