临床误诊误治Issue(5)：103-107,5.DOI:10.3969/j.issn.1002-3429.2015.05.034
原因不明习惯性流产患者肿瘤坏死因子-α和转化生长因子-β的表达及临床意义
Expressions of TNF-αand TGF-βin Women with Unknown Aetiology Recurrent Spontaneous Abortion and its Clinical Significance
摘要
Abstract
Objective To investigate the expression levels and clinical significance of TNF-α and TGF-β in women with unknown aetiology recurrent spontaneous abortion ( RSA) . Methods 60 women with unknown aetiology RSA admitted to our hos-pital during January 2011 and June 2013 were chosen as unknown aetiology RSA group and 70 normal pregnant women of artificial a-bortion admitted to our hospital during January 2011 and June 2013 were chosen as normal pregnant group. Enzyme-linked immu-nosorbent assay ( ELISA) was used to evaluate the protein levels of TNF-α and TGF-β in peripheral blood of unknown aetiology RSA group and normal pregnant group. The protein levels of TNF-αand TGF-βin villous and decidual tissues of unknown aetiology RSA group and normal pregnant group were tested using immunohistochemistry staining. Results ELISA result showed that the lev-el of TNF-αin peripheral blood of unknown aetiology RSA group was increased significantly as compared with that of normal preg-nant group [(213. 69 ± 4. 17)ng/ml vs. (135. 14 ± 5. 38)ng/ml, P<0. 05], whereas the level of TGF-βin peripheral blood of un-known aetiology RSA group was significantly lower than that of normal pregnant group [(240. 32 ± 3. 27) ng/ml vs. (403. 05 ± 6. 71)ng/ml, P<0. 05]. Immunohistochemistry staining result showed that the TNF-αprotein was expressed in villous issues of the two groups, and decidual cells of the two groups showed negative expression of TNF-α, the expression of TNF-αin villous tissues of unknown aetiology RSA group was significantly higher than that in normal pregnant group (P<0. 05);TGF-βexpression in the two groups were villus syncytiotrophoblast cells, the expression of TGF-βin villous tissues of unknown aetiology RSA group was signifi-cantly lower than that in normal pregnant group (P<0. 05);Decidual tissues of two groups showed weak positive expression of TGF-β, the expression of TGF-βin decidual tissues of RSA group was lower than that in normal pregnant group, however, there was no statistically significant difference between the two groups (P>0. 05). Conclusion The increase of expression of TNF-α and the expression of TGF-βin peripheral blood and decidual tissues may be involved in the pathogenesis of unknown aetiology RSA.关键词
流产,习惯性/肿瘤坏死因子-α/转化生长因子-βKey words
Abortion, habitual/TNF-α/TGF-β分类
医药卫生引用本文复制引用
高艳霞,杨波,孙前,李红敏..原因不明习惯性流产患者肿瘤坏死因子-α和转化生长因子-β的表达及临床意义[J].临床误诊误治,2015,(5):103-107,5.基金项目
河北省计生委课题(2011A07) （2011A07）
