临床小儿外科杂志Issue(2)：102-105,4.DOI:10.3969/j.issn.1671-6353.2015.02.006
AQP1在先天性巨结肠肠壁的表达及其意义
The expression and its significance of AQP-1 in the intestinal wall of Hirschsprung′s disease
摘要
Abstract
Objective To explain the possible pathogenesis of ENS defection in HD,we now reveal the abnormal expression of a water channel protein,which named Aquaporin—1(AQP—1),in intestinal wall of Hir-schsprung's disease and the potential role AQP—1 played in the development of HD.Methods Specimens were collected from resected colon in both aganglionic bowel segments and ganglionic segments of 65 HD patients. The Colon in the control group colon was resected from 21 age-matched patients without HD.All specimens un-derwent haematoxylin and eosin(HE)staining,followed by immunostaining for AQP—1、Cathepsin D and S—100. Positively stained area density was evaluated at 400 magnification,calculating the sum of the areas which occu-pied on the basis of the positively stained area in plexuses.Results ①The ganglionic cells were detected in the dilated Segments of HD and tissue of control group,which is failed in narrow segments by HE,also in immu-nohistochemical staining of Cathepsin-D and S — 100;②AQP — 1 was strongly positive in Endothelial cells both within the aganglionic and ganglionic gut and control group.③In the ganglionic cells and Schwann Cells of the dilated segments,AQP — 1 was positive both in the Auerbach plexuses and Meissner plexuses but negative in most of(about 90.2%)the narrow group.④Semi-quantitative analysis showed that the positive staining area percentage of AQP— 1 has significant differences between the narrow segments and the dilated segments of HD (P <0.001). Conclusions AQP — 1 immunohistochemical staining was strongly positive in ganglionic cells both in the dilated segments group and the normal colon but mostly negative in the narrow segments group.We presumably that AQP—1 may play a important role in the intestinal nerve cells migration to induce the develop-ment of HD thus AQP— 1can be useful as one of the neuronal markers for the diagnosis of HD.关键词
Hirschsprung 病/水通道蛋白质 1/免疫组织化学Key words
Hirschsprung Disease/Aquaporin 1/Immunohistochemistry引用本文复制引用
甘亮,杨文萍,张守华,徐红艳,曾华,陶俊峰,陈快,黄金狮,陶强..AQP1在先天性巨结肠肠壁的表达及其意义[J].临床小儿外科杂志,2015,(2):102-105,4.基金项目
江西省卫生计生委基金资助项目 （）
