临床与实验病理学杂志Issue(4)：393-396,4.DOI:10.13315/j.cnki.cjcep.2014.04.009
肝癌细胞系L2不同途径接种造模的比较
Comparison of liver cancer models established by different injection routes of rat L2 hepato-cellular carcinoma cells
摘要
Abstract
Purpose To compare and investigate the oncogenic and metastatic phenotypes in nude mice by injection of rat hepatocellu-lar carcinoma ( HCC) cells L2 via two different routes. Methods Twenty of 7-week-old female BALB/cA mice were randomly divided into 2 groups. After the injections of L2 from liver or spleen lobe, the survival rate, tumor formation rate, carcinogenic features, and metastasis were comparatively studied. Results All of the liver orthotopic nude mice developed liver cancer (100%) with 60% lung metastasis rate, and exhibited an expansive growing pattern with surrounding invasion. In the spleen orthotopic model, 78% mice de-veloped HCC in spleen, with 67% liver metastatic rate and 11% lung metastatic rate, lower than the liver orthotopic model ( P <0. 05). But the microscopically hilar lymph node metastasis rate was 33%. Conclusion The direct liver injection of L2 in female nude mice is a better modeling method for studying the mechanism of both carcinogenesis and metastasis, as well as the evaluation of therapeutic effect of liver cancer.关键词
肝肿瘤/肝癌细胞系L2/转移/裸鼠/动物模型Key words
liver neoplasms/hepatocellular carcinoma cells L2/metastasis/nude mice/animal model分类
医药卫生引用本文复制引用
刘勇,唐红,罗霞,阮思蓓,张元,唐明希..肝癌细胞系L2不同途径接种造模的比较[J].临床与实验病理学杂志,2014,(4):393-396,4.基金项目
四川省教育厅重点项目(08ZA090)、四川省科技厅应用基础项目(2010JY0130)、泸州医学院博士启动基金(2011204)、泸州医学院科研启动基金(2012-LG-03)、泸州医学院自然科学基金(55) （08ZA090）
