临床与实验病理学杂志Issue(4)：397-400,4.DOI:10.13315/j.cnki.cjcep.2014.04.010
缬沙坦对糖尿病小鼠肾小球组织中Notch信号通路表达及细胞外基质生成的影响
Effect of valsartan on Notch pathway and extracellular matrix in glomeruli of diabetic mice
摘要
Abstract
Purpose To investigate effect of valsartan on expression of Notch pathway and production of extracellular matrix. Methods Urine protein and production of extracellular matrix were measured after diabetic mice were treated with valsartan. The levels of Jag-ged1, Notch1, Notch intracellular domain 1 (NICD1), transforming growth factor-β1 (TGF-β1), TypeⅣcollagen and Laminin were determinated by immunohistochemistry, Western blot and real-time PCR. Results Valsartan suppressed urine protein and production of extracellular matrix in diabetic mice (P<0. 05). Valsartan also inhibits overexpression of Jagged1, Notch1, NICD1, TGF-β1, Type Ⅳ collagen and Laminin in glomerular tissues of diabetic mice (P<0. 05 or P<0. 01). Conclusion Valsartan inhibits activa-tion of Notch pathway in glomerular tissues of diabetic mice, inhibits production of extracellular matrix in glomerulus and delays glomer-ulosclerosis.关键词
糖尿病肾病/Notch信号通路/细胞外基质/缬沙坦/小鼠Key words
diabetic nephropathy/Notch pathway/extracellular matrix/valsartan/mice分类
医药卫生引用本文复制引用
王晓梅,丁洋,赵玉峰,郝军,高峰..缬沙坦对糖尿病小鼠肾小球组织中Notch信号通路表达及细胞外基质生成的影响[J].临床与实验病理学杂志,2014,(4):397-400,4.基金项目
河北省医学科学研究重点课题(20130229) （20130229）
