临床与实验病理学杂志Issue(9)：958-962,5.DOI:10.13315/j.cnki.cjcep.2014.09.003
乳腺癌中PKCζ、MMP-2、MMP-9的表达及相关性
Expression and correlation of PKCζ, MMP-2 and MMP-9 in breast cancer
摘要
Abstract
Purpose To investigate the expressions of PKCζ, MMP-2, and MMP-9 in breast cancer and the relationship with the inva-sion and metastasis of breast cancer. Methods The expression of PKCζ, MMP-2 and MMP-9 in 100 cases with breast cancer was as-sessed with immunohistochemistry PV 9000 method. PKCζ-siRNA was transfected into MDA-MB-231 cell lines, called siPKCζ/MDA-MB-231. While siRNA construct containing a scrambled sequence was transfected into MDA-MB-231 cells to generate control cells, which were designated as Scr/MDA231 cells. Western blotting was used to measure the expression of PKCζ in transfected cells, and the Transwell invasion assay was used to detect the invasion ability in vitro. The content of MMP-2, MMP-9 were measured by ELISA. Results The expression rates of PKCζ, MMP-2 and MMP-9 in breast cancer tissues were 62.5%, 37.5% and 32.5%, and there were significant differences among them (P<0.05). The expression of PKCζwas much higher than those in the normal breast tissues nearby. The expression of PKC protein was assoiated with lymph node metastasis, distant metastasis (P<0.01), but was not correla-ted with other clinicopathologic parameters, such as age, tumor size, histological type, ER, PR, and so on (P>0.05). The expres-sion of PKCζ, MMP-2 and MMP-9 were lower in siPKCζ/ MDA-MB-231 group than those in scr/ MDA-MB-231 group, and the in vitro invasion ability was significantly decreased (P<0.05). Conclusions PKCζ can promote the invasion and metastasis of breast canc-er, and correlated with the expression of MMP-2, MMP-9(P<0.05).关键词
乳腺肿瘤/PKC灼/MMP-2/MMP-9/侵袭/转移Key words
breast neoplasm/PKCζ/MMP-2/MMP-9/invasion/metastasis分类
医药卫生引用本文复制引用
杨硕,李洪利,李文通,杨璐,倪明,翟丽敏,尹崇高,张宝刚..乳腺癌中PKCζ、MMP-2、MMP-9的表达及相关性[J].临床与实验病理学杂志,2014,(9):958-962,5.基金项目
国家自然科学基金(81072068)、山东省中青年科学家奖励基金(2010BSB14050、BS2011YY060)、山东省高等学校科技计划项目(J12LK03、J13LK03、J10LF64)、潍坊医学院科技创新研究基金青年基金(K11QC1002) （81072068）
