临床与实验病理学杂志Issue(9)：971-974,4.DOI:10.13315/j.cnki.cjcep.2014.09.006
mTOR在乳腺癌中的表达及其抑制剂对乳腺癌细胞增殖和凋亡的作用
Expression of mTOR in breast cancer and the effect of mTOR inhibitor on the cell prolofera-tion and apoptosis of breast cancer cells
摘要
Abstract
Purpose To investigate the expression of mTOR in breast cancer, and to observe the effect of CCI-779 on proliferation and apoptosis of MDA-MB-231 cell. Methods Immunohistochemical staining was used to detect the expression of mTOR protein in breast cancer tissue and MDA-MB-231 cell. MTT method was used to show effect of CCI-779 on proliferation of MDA-MB-231 cell. Annex-inV-FITC/PI method was used to show effect of CCI-779 on apoptosis of MDA-MB-231 cell. Results 54.9% in 71 cases of breast cancer tissue could express mTOR protein, the expression was significantly higher than in 32 cases of normal tissue (21.9%), mTOR protein was also detected in MDA-MB-231 cell, CCI-779 could inhibit the proliferation of MDA-MB-231 cell, and show a dose-and time-dependent, but CCI-779 could not induce apoptosis of MDA-MB-231 with AnnexinV-FITC/PI assay. Conclusion mTOR is closely related to the formation of breast cancer, CCI-779 has strong activity against MDA-MB-231 cell, it has prospect for treatment of breast cancer in the future.关键词
乳腺肿瘤/CCI-779/mTOR/mTOR抑制剂Key words
breast neoplasm/CCI-779/mTOR/mTOR inhibitor分类
医药卫生引用本文复制引用
李媛媛,田红艳,刘雨清..mTOR在乳腺癌中的表达及其抑制剂对乳腺癌细胞增殖和凋亡的作用[J].临床与实验病理学杂志,2014,(9):971-974,4.基金项目
潍坊市科学技术发展计划(201302090) （201302090）
