临床与实验病理学杂志Issue(9)：975-978,4.DOI:10.13315/j.cnki.cjcep.2014.09.007
人子宫内膜癌中p57 kip2、Cyclin D1的表达及意义
Expression and significance of p57 kip2, Cyclin D1 in human endometrial carcinoma
摘要
Abstract
Purpose To study the effects of p57kip2, Cyclin D1 on the carcinogenesis and progression of endometrial carcinoma. Meth-ods 100 cases of endometrial adenocarcinoma ( EA) , 20 cases of endometrial intraepithelial neoplasia ( EIN) , 20 cases of hyperpla-sia lesion, 20 cases of endometrial proliferative phase were selected. Different endometrial cells ( well-differentiated endometrial cancer cells Ishikawa, moderately differentiated endometrial cancer cells JEC, poorly differentiated endometrial cancer cells KLE and normal endometrial cells ESC) were cultured. Protein expression of p57kip2, Cyclin D1 was measured by immunohistochemical EliVision meth-ods. The expression of p57kip2, Cyclin D1 protein in different endometrial cells was detected by Western blot. Results The highest expression of p57kip2 protein was in EIN, the higher expression was in endometrial proliferative phase, the low expression was in EA and the lowest expression was in hyperplasia lesions, but only the expression of p57kip2 protein in EIN was higher than that in hyperplasia le-sions (P<0.05). The highest expression of Cyclin D1 protein was in EA and the lowest expression of Cyclin D1 protein was in endom-etrial proliferative phase, expression of Cyclin D1 protein increased gradually in hyperplasia lesions and EIN, p57kip2, Cyclin D1 pro-tein expression in the tissue of EA increased along with the histological grade, all present decreasing trend, but only p57kip2 protein ex-pression related to histological grade ( P<0.05 ) . The highest expression of p57 kip2 protein was in KLE and the lowest expression of p57kip2 protein was in ESC, but only the expression of p57kip2 protein in KLE was higher than that in ESC (P<0.05). The expression of Cyclin D1 in JEC, Ishikawa higher than that in ESC (P<0.05). Conclusion p57kip2, Cyclin D1 are involved in the occurrence and development of endometrial carcinoma, Cyclin D1 is an early event in endometrial carcinoma, but there may also be have abnormal p57kip2 protein by synthesized, synergistically. Cyclin D1 promoting endometrial malignant transformation. Combined detection of p57kip2, Cyclin D1 expression in endometrial carcinoma, to predict the prognosis of endometrial carcinoma has a certain clinical signifi-cance.关键词
子宫肿瘤/子宫内膜癌/子宫内膜样腺癌/p57 kip2/Cyclin D1/免疫组织化学Key words
uterine neoplasm/endometrial carcinoma/endometrial adenocarcinoma/p57 kip2/Cyclin D1/immunohistochemistry分类
医药卫生引用本文复制引用
格桑志玛,周正平,罗祖强,肖庆邦,孙小杰..人子宫内膜癌中p57 kip2、Cyclin D1的表达及意义[J].临床与实验病理学杂志,2014,(9):975-978,4.基金项目
贵州省教育厅自然科研基金(黔教科2010047)、贵州省社发攻关项目[黔科合SY字(2013)3011 （黔教科2010047）
